牵张应力环境下六味地黄汤含药血清对成骨细胞增殖分化影响的实验研究  

Influence of serum containing Liuwei Dihuang decoction(六味地黄汤) on proliferation of osteoblasts under stretch-stress environment

作　　者：程婉[1] 汤小康[1] 应航[1] 李敏[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中国骨伤》2013年第2期142-146,共5页China Journal of Orthopaedics and Traumatology

基　　金：浙江省中医药科学研究基金计划(编号:2010ZA020);浙江省自然科学基金(编号:Y2110928)~~

摘　　要：目的:探讨不同时间、不同形变量的牵张应力环境下,六味地黄汤含药血清对体外培养的新生SD大鼠成骨细胞增殖分化的作用。方法:制备血清后,将六味地黄汤含药血清及空白对照血清分别作用于2组成骨细胞24h,然后采用频率0.5Hz、形变量分别为6%和12%的牵张应力,对六味地黄汤含药血清组及空白血清对照组的成骨细胞进行力学加载,分别于12、24h行碱性磷酸酶(ALP)活性测定(检测各组碱性磷酸酶活性)和MTT检测(检测受力后各组成骨细胞增殖的情况),并利用统计学软件对相关数据进行分析。结果:①在牵张应力环境下以0.5Hz的频率牵拉成骨细胞24h,6%与12%的形变量均能刺激成骨细胞增殖与分化,其中12%的形变量作用优于6%的形变量。②六味地黄汤含药血清短时间刺激对体外培养的SD大鼠成骨细胞尚未见促进增殖分化作用。结论:牵张应力环境能促进体外培养的成骨细胞增殖和分化,短时间六味地黄汤含药血清作用对成骨细胞影响不明显。Objective ：To study influence of serum containing Liuwei Dihuang decoction （六味地黄汤）on proliferation and differentiation of osteoblast form neonatal SD rats cultured in vitro at different times and different stretch stress. Methods： After osteoblast cultured for 24 hours in the serum containing Liuwei Dihuang decoction （六味地黄汤 ）and serum in control group,the 0.5 Hz frequency,6% and 12% stretch-stress were added. The MTF and the activity of ALP were measured at the 12th and 24th hours, and the data were analyzed. Results：①In the environment of stretch stress to the frequency of 0.5 Hz, and stretched for 24 hours ,the osteoblast was stimulated under elongation rate of 6% and 12%; the proliferation and differenti- ation of osteoblast was more active under elongation rate of 12% than that of 6%.②There were no stimulating effects on os- teoblast proliferation and differentiation of serum containing Liuwei Dihuang decoction （六味地黄汤 ）acted on osteoblast cells of SD rats cultured in vitro for a shot time. Conclusion： Stretch stress environment can enhance osteoblast proliferation and dif- ferentiation cultured in vitro.

关 键 词：应力 物理 成骨细胞 细胞增殖 补肾药 

分 类 号：R681[医药卫生—骨科学]