盐酸米托蒽醌诱导人鼻咽癌细胞CNE-2凋亡的研究  

Effect of apoptosis induced by mitoxantrone on human nasopharyngeal carcinoma cell line CNE-2

作　　者：王炜[1] 刘斌[2] 童春义[2] 程小广[1] 陈稚[1] 赵毅[1] 胡新荣[1] 

机构地区：[1]广东医学院分析中心,广东东莞523808 [2]湖南大学生物学院,湖南长沙410082

出　　处：《中外医疗》2013年第2期15-17,共3页China & Foreign Medical Treatment

基　　金：东莞市高校项目(2011108102026);广东医学院青年基金资助

摘　　要：目的探讨盐酸米托蒽醌诱导人鼻咽癌细胞CNE-2凋亡作用和机制。方法用CCK-8细胞增殖及细胞毒性检测试剂盒检测盐酸米托蒽醌对CNE-2细胞生长的抑制作用,流式细胞仪检测细胞内药物积聚量与细胞凋亡率之间的关系,共聚焦检测盐酸米托蒽醌在细胞中的定位。结果盐酸米托蒽醌对CNE-2细胞生长具有抑制作用,呈时间和剂量依赖性,并能有效诱导细胞凋亡,其在细胞中主要以斑点形式分布在细胞质中。结论盐酸米托蒽醌能抑制人鼻咽癌细胞CNE-2细胞生长,并诱导细胞凋亡,CNE-2细胞的药物敏感性与药物在细胞内的分布位置有直接关系。Objective To investigate the effect of mitoxantrone on nasopharyngeal carcinoma CNE-2 cells＇ apoptosis. Methods The effects of mitoxantrone on the proliferation of CNE-2 cells were detected by CCK-8 cell proliferation and cytotoxicity assay kit.The relationship between intracellular drug accumulation and cell apoptosis was detected by flow cytometry.The distribution of mitoxantrone in CNE-2 cells was detected by confocal microscope. Results Mitoxantrone had strong toxicity on CNE-2 cell in a dose and time-dependent manner,it mainly in the form of distribution in the cytoplasm. Conclusion Mitoxantrone can inhibit the growth and induce apoptosis in CNE-2 cell lines .The chemosensitity of CNE-2 was directly related With intracellular distribution of Mitoxantrone.

关 键 词：盐酸米托蒽醌 鼻咽癌 生长抑制 凋亡 

分 类 号：R739[医药卫生—肿瘤]