何首乌丛枝菌根真菌的多样性  

Diversity of Arbuscular Mycorrhizal Fungi of Polygonum multiflorum

作　　者：罗充[1] 吴涛[1] 谭金玉[1] 

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550001

出　　处：《贵州农业科学》2013年第2期107-111,共5页Guizhou Agricultural Sciences

基　　金：贵州省自然科学基金"菌根真菌在西南岩溶地区退化生态系统修复中的作用研究"[黔科合J字(2007)2048];贵州师范大学资助博士科研项目"丛枝菌根真菌对黔产道地药材有效成分的诱导效应及机制研究"(11904-05032100010)

摘　　要：为探索贵阳市东山何首乌丛枝菌根(Arbuscular Mycorrhizal,AM)真菌的种类及多样性,为优势AM真菌在何首乌人工栽培上应用提供理论依据,采用酸性品红染色法和湿筛法测定何首乌丛枝菌根真菌的侵染率,分离何首乌根际土壤中的AM真菌孢子并进行形态学鉴定和多样性分析。结果表明:何首乌根系的菌根侵染率为7.5%;从根际土壤中分离到AM真菌4个属55种和一个未鉴定种,其中:球囊霉属(Glomus)31种,无梗囊霉属(Acaulospora)22种,盾巨孢囊霉属(Scutellospora)1种,多孢囊霉属(Diversis-pora)1种,优势种主要为枫香球囊霉(G.liquidambaris)、褐色球囊霉(G.badium)、孢果无梗囊霉(A.sporocarpia)。东山何首乌AM真菌资源丰富,球囊霉属和无梗囊霉属的适应性强、分布广泛。To explore the type and diversity of arbuscular mycorrhizal fungi（AMF） of P. multiflorum in Dongshan, Guiyang, and to provide a theoretical evidence for the dominant species on the artificial cultivation of application. By using acid fuchsin staining and wet-screening method, the root colonization rate of the roots of P. multiflorum were measured, and identified by their morphological characteristics and diversity analysis. The result showed that the root colonization rate of P. multiflorum was 7.5%. 55 species of four genera and one species not identifyied were isolated from the rhizospheres, including 31 species of Glornus, 22 species of Acaulospora, one species of Scutellospora, and one species of Diversispora. G. liquidambaris, G. badiurn and A. sporocarpia were the dominant species. The AMF resources were rich in P. multiflorurn, Glornus and Acaulospora were of strong adaptability and wide distribution in Dongshan area.

关 键 词：何首乌 丛枝菌根真菌 多样性 

分 类 号：S154.3[农业科学—土壤学]