系统性红斑狼疮患者外周血CD19^+细胞数量的变化及p16 mRNA的表达  

The quantity of CD19^+ lymphocytes and the expression of pl6 mRNA in peripheral blood of patients with systemic lupus erythematosus

作　　者：马红[1] 许辉[1] 张雪静[1] 闵敏[1] 李遇梅[1] 

机构地区：[1]江苏大学附属医院皮肤科,江苏镇江212000

出　　处：《中国麻风皮肤病杂志》2013年第2期79-81,共3页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金资助项目(编号:30771938)

摘　　要：目的:检测系统性红斑狼疮患者外周血中CD19^+细胞数目的变化及p16 mRNA的表达。方法:采用免疫磁珠法分离SLE外周血CD19^+细胞并计数;分选出来的细胞提取RNA,应用实时定量RT-PCR方法检测CD19^+细胞中p16 mRNA表达水平。结果:SLE患者外周血中CD19^+细胞(66.23±4.455)较正常对照组(32.15±5.108)增加。SLE患者p16 mRNA在CD19^+细胞中的表达(47.3731±22.2939)比健康对照组(0.7276±0.1183)明显增加。结论:CD19^+细胞的数量及p16 mRNA的异常在SLE的发病中起一定作用。Objective： To assess the quantity of peripheral blood of CD19＋ ceils and detect the expression of p16 mRNA on peripheral blood of CD19＋ ceils in the patients with systemic lupus erythematosus （SLE）. Methods： CD19＋ cells were separated from the peripheral blood using immunomagnetic beads and counted in 30 SLE patients and 20 hea/thy controls. Reverse transcription - pelymerase chain reaction （RT - PCR） technique was applied to quantitatively analyze the expression of p16 mRNA in CD19＋cells. Results： Compared with controls, the quantity of CD19＋ cells and the level of p16 mRNA expression in CD19＋ cells were significantly increased in SLE patients. Conclusion： The abnormality in the quantity of CD19＋ cells and the expression of p16 may play a role in the patho- genesis of SLE.

关 键 词：P16 MRNA 系统性红斑狼疮 CD19+ 

分 类 号：R593.24[医药卫生—内科学]