甲状腺素对甲状腺功能减低大鼠子代脑组织中同源盒基因Nkx6．2表达的影响  

Effects of Thyroid Hormone on the Expression of Homeobox Gene Nkx6.2 in the Cerebrum Tissue of Offspring of Hypothyroidism Pregnant Mother

作　　者：张欣[1] 汪蓓蕾[1] 张瑞[1] 郭刚[1] 

机构地区：[1]天津医科大学代谢病医院内分泌研究所、卫生部激素与发育重点实验室,300070

出　　处：《天津医药》2013年第2期139-141,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30571617)

摘　　要：目的探讨甲低大鼠妊娠不同时间补充不同剂量甲状腺素(L-T4)对子代大鼠脑组织中同源盒基因Nkx6.2 mRNA表达的影响。方法120只雌性Wistar大鼠分为对照组,甲低组,甲低孕鼠妊娠早期(1～17d)补充L-T4高、中、低剂量组,甲低孕鼠妊娠晚期(18～20d)补充L-T4高、中、低剂量组,每组15只,与正常雄性大鼠交配后分别取孕17d、新生期、20d龄仔鼠脑组织,采用实时荧光定量PCR法检测脑Nkx6.2 mRNA表达。结果甲低组与对照组对应日龄仔鼠脑Nkx6.2 mRNA表达差异有统计学意义(均P<0.01);除甲低孕早期中、高剂量组与对照组对应日龄仔鼠的差异及甲低孕晚期高剂量组新生期仔鼠与同日龄对照组的差异无统计学意义外(均P>0.05),其他补充L-T4组与对照组同日龄仔鼠的差异均有统计学意义(均P<0.01)。结论甲低孕鼠子代大鼠脑组织同源盒基因Nkx6.2mRNA的表达受补充甲状腺素剂量及时间影响。Objective To explore the effects of thyroid hormone and complement of levothyroxine （L-thyroxine, L-T,） to pregnant mother on the expression of homeobox gene Nkx6.2 mRNA in rat offspring. Methods One hundred and twenty Wistar rats were randomly divided into normal control group, hypothyroidism group, hypothyroidism in early pregnancy group （1-17 d） supplied with high dosage, medium dosage and low dosage L-T4 and hypothyroidism in late pregnancy group （18-20 d） supplied with high dosage, medium dosage and low dosage L-T4 （15 rats for each groups）. The brain tissues of offspring were taken at gestation dayl7, postnatal day 0 and postnatal day 20, and the content of Nkx6.2 mRNA was detected by re- al-time fluorescence quantitative PCR techniques. Results There was significant difference in Nkx-6.2 expression level be- tween hypothyroidism group and normal control group （P 〈 0.01）. There were significant differences in Nkx-6.2 expression levels between groups with supplement of high dosage, medium dosage and low dosage L-T4 and control group （P 〈 0.01）. Conclusion The expression of Nkx6.2 mRNA is highly related to the supplemental thyroxine dosage and time impact in hy- pothyrodism preganant rats.

关 键 词：甲状腺功能减退症 甲状腺功能试验 基因 同源盒 甲状腺素 聚合酶链反应 疾病模型 动物 大鼠  Wistar 

分 类 号：R827.14[医药卫生—临床医学]