复方丹参对犬牙齿移植作用的免疫组织化学研究  

Immunohistochemical Study on the Effect of the Compound Salvia on Canine Tooth Transplantation

作　　者：张海燕 彭利伟[2] 

机构地区：[1]郑州市口腔医院修复种植科,河南郑州450000 [2]河南省人民医院口腔科

出　　处：《口腔医学研究》2013年第2期128-131,共4页Journal of Oral Science Research

基　　金：河南省自然科学基金资助项目(0611045500);河南省三门峡市杰出青年基金资助项目(051073102);河南省卫生厅科技攻关资助项目河南省卫生科技创新型人才工程专项经费资助(4022)

摘　　要：目的:研究复方丹参在犬牙齿自体移植中的作用,对犬移植牙齿牙周组织的影响及可能的机制。方法:5只成年雄性杂种犬的移植牙齿分为实验组、对照组,对犬牙齿移植后1周、2周、3周、1个月共4个时间点的移植牙的牙体-牙周联合标本进行组织学HE染色;PV二步法检测TGF-β1和PCNA的表达情况。结果:实验组牙周组织改建活跃,TGF-β1和PCNA的阳性表达明显高于对照组,两组比较存在统计学差异(P<0.001)。结论:复方丹参可提高牙周组织中TGF-β1和PCNA的表达,这可能是其对犬移植牙作用的机制之一。[Abstract] Objective： To study the effect of Compound Salvia on canine tooth autotransplantation, and possible mechanisms of Compound Salvia on periodontal tissue of autotransplanted teeth. Methods： Transplanted teeth from five adult male hybrid dogs were selected, which were divided into two groups： the experiment group and the con- trol group. The teeth - periodontal joint specimens were stained with HE and＇ immunohistochemical staining by the two-step powervisionTM（PV） to detect the expression of TGF-β1 and PCNA 4 times, respectively after the transplantation had been conducted for 1 week, 2 weeks, 3 weeks and 1 month. Results： Compared with the control group, the reconstruction of the periodontal tissue of the transplanted teeth of the experimental group was more ac- tive. The positive expression of the experimental group＇s TGF-β1 and PCNA was significantly higher than that of the control group. There was a statistical difference between the experimental group and the control group （P〈0. 001）. Conclusion： Compound Salvia can improve the expression of TGF-β1 and PCNA in the periodontal tissue of transplanted teeth, which may be one of effect mechanisms of Compound Salvia on tooth transplantation.

关 键 词：复方丹参 牙齿移植TGF-β1 PCNA成骨细胞 免疫组化 

分 类 号：R285.5[医药卫生—中药学]