EV71不同接种途径对BALB／c小鼠的感染特点  

Study on BALB/c mice with EV71 infection by diverse inoculation routes

作　　者：李鹏[1] 岳盈盈[1] 宋楠楠[1] 李志会[1] 孟红[1] 

机构地区：[1]山东省医学科学院基础医学研究所,山东省罕少见病重点实验室,济南250062

出　　处：《中华实验和临床病毒学杂志》2013年第1期14-17,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金资助项目（81000720）;山东省自然科学基金资助项目（ZR2011HQ030）

摘　　要：目的研究EV71JN200804株对1日龄BALB／c小鼠的感染特点，建立EV71感染BALB／c小鼠的动物模型，为疫苗和抗病毒药物的研究提供可靠的动物评价工具。方法EV71JN200804株分别采用口服、颅内注射、肌内注射和腹腔注射感染1日龄BALB／e小鼠，出现后肢麻痹后，做后肢电生理检测；安乐动物，采集脑、脊髓、心脏、肝脏、脾脏、肺脏、肾脏、胸腺、小肠及后肢肌肉，进行动物体内的病毒分离和RT-PCR鉴定，同时对各器官组织进行组织病理学观察。结果接种EV71后，颅内、肌内和腹腔注射组小鼠体重增长缓慢，4～5d出现后肢麻痹，7d左右死亡。RT—PCR和病毒分离表明颅内、肌内和腹腔注射组的肌肉分离到病毒，颅内注射组脊髓也分离到病毒，经RT—PCR鉴定为EV71感染。肌电图显示颅内、肌内和腹腔注射组的时限显著增加，波幅显著降低，判断小鼠后肢麻痹可能既有神经源性损害又有肌源性损害。组织病理学观察发现，颅内、肌内、腹腔注射组小脑浦肯野细胞及颗粒细胞减少，脊髓前角白质区神经纤维肿胀，后肢肌肉组织大片坏死溶解、炎性细胞浸润，肺组织明显充血，局部心肌细胞肿胀，部分肝组织内可见巨噬样细胞及淋巴样细胞浸润，部分肾皮质中肾小球萎缩、数目减少，而口服组无明显病理变化。结论EV71JN200804株通过颅内、肌内和腹腔注射三种途径均能感染并导致1日龄BALB／c小鼠后肢麻痹，此动物模型可用于EV71致病机制和特异性抗病毒药物的研究及疫苗的评价。Objective To study the characteristics of EV71 JN200804 strain infection in one-day old BALB/c mice and to establish a animal model of EV71 infection, and to provide information and technical support for the evaluation of the EV71 vaccine and antiviral medicine. Methods One-day old BALB/c mice were infected with EV71 JN200804 strain through oral（PO） ,intracranial （IC） , intraperitoneal （IP）, intramuscular （IM） routes, respectively. All mice were sacrificed at paralysis of hind limbs and collected organs for viral isolation, RT-PCR and pathological examination, and the electrophysiology were detected before sacrifice. Results All mice infected through IC, IP and IM routes were paralyzed in hind limbs at 4-5 days and died at 7 days about, the hypokinesia and lethargy of mice were observed through PO routes. The viruses could be isolated and detected in the muscle from mice infected through IC, IP and 1M routes and in the spinal cord through IC routes by viral isolation and RT-PCR. The neurogenic and myogenic disorders were detected by electromyography. Histopathologically, the varied pathologic changes were observed in the mouse cerebellum, spinal cord, muscle, heart, lung, liver and kidney. Conclusion EV71 JN200804 strain can infect one-day old BALB/c mice and induce paralysis of hind limbs, its animal infection model may apply to study of EV71 infection pathogenesis and antiviral medicine, and evaluation of the EV71 vaccine.

关 键 词：肠道病毒属 小鼠 近交BALB c 模型 动物 

分 类 号：R725.1[医药卫生—儿科]