肿瘤坏死因子-α和基质金属蛋白酶-9在儿童及成人中耳胆脂瘤中的表达及意义  

Expression and significance of tumor necrosis factor-α and matrix metalloproteinase-9 in middle ear cholesteatoma of children and adults

作　　者：汪志伟[1,2] 杨海弟[3] 赵晓明[1,2] 窦晓辉[1,2] 李永贺[4] 

机构地区：[1]珠海市人民医院 [2]暨南大学附属珠海医院耳鼻咽喉科,广东珠海519000 [3]中山大学孙逸仙纪念医院耳鼻咽喉科,广东广州510120 [4]南方医科大学珠江医院耳鼻咽喉科,广东广州510280

出　　处：《中国耳鼻咽喉头颈外科》2013年第2期99-101,共3页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的探讨肿瘤坏死因子-α(tumor necrosisfactor-α,TNF-α)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在儿童及成人中耳胆脂瘤中的表达差异和临床意义。方法术中观察22例儿童及36例成人胆脂瘤型中耳炎患者听骨链破坏情况。采用免疫组化技术检测TNF-α和MMP-9在患者胆脂瘤中的表达。分析不同年龄组患者听骨链破坏程度和TNF-α、MMP-9表达差异,以及TNF-α、MMP-9表达的相互关系。结果儿童胆脂瘤型中耳炎患者听骨链破坏程度高于成人患者。TNF-α在儿童及成人中耳胆脂瘤中的相对吸光度值分别为33.32±3.90和26.61±4.43,MMP-9在儿童及成人中耳胆脂瘤中的相对吸光度值分别为35.50±3.33和27.25±3.83,两者在儿童的表达均明显强于成人,且两者在不同年龄组患者中的表达均呈正相关。结论 TNF-α和MMP-9在儿童中耳胆脂瘤中的表达强于成人患者,可能是儿童胆脂瘤更具破坏力和侵袭性的重要原因。OBJECTIVE To investigate the difference of expression and clinical significance of tumor necrosis factor-a （TNF-9） and matrix metallo proteinase-9 （MMP-9） in middle ear cholesteatoma of children and adults. METHODS The destruction of auditory ossicles of 22 children and 36 adults with middle ear cholesteatoma was observed in operations. The expressive levels of TNF-a and MMP-9 in middle ear cholesteatoma of these patients were examined by immunohistochemical method. The destruction of auditory ossicles, the difference and the correlation of expression between TNF-a and MMP-9 in patients of different ages were analyzed. RESULTS The destruction of auditory ossicles in children with cholesteatoma was more severe than that of adults. The corresp optical density （COD） of TNF-a in cholesteatoma of children and adults were 33.32±3.90 and 26.61 ±4.43, the COD of MMP-9 in cholesteatoma of children and adults were 35.50± 3.33, 27.25±3.83. The expressive levels of TNF-a and MMP-9 in children were both higher than that in adults. The expressions of TNF-a and MMP-9 were positively correlated in both children and adults. CONCLUSION The expressive levels of TNF-a and MMP-9 in cholesteatoma of children are higher than that of adults. It may be the important reason that the cholesteatoma of children is more destructive and more invasive than that of adults.

关 键 词：肿瘤坏死因子类 明胶酶B 胆脂瘤 中耳 儿童 成年人 

分 类 号：R764[医药卫生—耳鼻咽喉科]