姜黄素对糖尿病早期大鼠视网膜Müller细胞的保护作用  

Protective effects of curcumin on retinal Müller cell in early diabetic rats

作　　者：谷学静[1] 左中夫[1] 

机构地区：[1]辽宁医学院人体解剖学教研室,辽宁省锦州市121001

出　　处：《眼科新进展》2013年第3期233-235,共3页Recent Advances in Ophthalmology

摘　　要：目的探讨姜黄素对糖尿病早期大鼠视网膜Müller细胞的影响及作用机制。方法雄性SD大鼠腹腔注射链脲佐菌素诱导糖尿病(diabetes mellitus,DM)模型,实验分4组(每组各6只):对照组(SD大鼠腹腔单次注射柠檬酸缓冲液)、DM组(链脲佐菌素诱导的DM大鼠)、DMSO组(DM大鼠腹腔注射二甲基亚砜及生理盐水混合液,每天一次)、姜黄素组(DM大鼠腹腔注射姜黄素80mg·kg(-1),每天一次)。3个月后检测视网膜丙二醛(malondialdehyde,MDA)及还原型谷胱甘肽(reduced gluta-thione hormone,GSH)含量,视网膜胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)免疫组织化学染色,Western-blot检测视网膜GFAP及谷氨酰胺合成酶(glutamine synthetase,GS)表达变化,高效液相色谱检测视网膜谷氨酸含量。结果对照组MDA、谷氨酸、GSH分别为(3.47±0.51)nmol·mg(-1)prot、(35.6±4.8)nmol·mg(-1)、(98.61±9.82)mg·g(-1)prot,DM组分别为(7.21±0.83)nmol·mg(-1)prot、(49.4±4.1)nmol·mg(-1)、(54.86±3.47)mg·g(-1)prot,DMSO组分别为(7.13±0.74)nmol·mg(-1)prot、(52.5±3.7)nmol·mg(-1)、(59.61±5.38)mg·g(-1)prot;DM组及DMSO组MDA及谷氨酸含量较对照组明显增加,GSH含量降低,差异均有统计学意义(均为P<0.05);姜黄素组MDA、GSH及谷氨酸含量分别为(3.91±0.43)nmol·mg(-1)prot、(86.93±7.84)mg·g(-1)prot、(38.4±3.7)nmol·mg(-1),与对照组相比差异均无统计学意义(均为P>0.05),而与DM组相比,姜黄素组MDA含量、谷氨酸含量均降低(均为P<0.05),GSH含量增加(P<0.05)。Western-blot检测结果显示,DM及DMSO组视网膜GFAP表达上调,GS表达下调,姜黄素组GFAP及GS表达均无明显变化。免疫组织化学染色结果显示,对照组视网膜Müller细胞未见明显GFAP阳性染色,DM组及DMSO组Müller细胞胞体及突起呈明显GFAP阳性,但姜黄素组Müller细胞GFAP阳性染色明显弱于DM组。结论姜黄素可上调DM早期大鼠视网膜GS表达,清除视网膜谷氨酸,保护Müller细胞,其机制可能�Objective To explore the effects and potential mechanisms of curcu- min （CUR） on retinal Mtiller cell in early diabetic rats. Methods Diabetes mellitus （DM） models were induced by single intraperitoneal injection of streptozotocin（STZ）. Male SD rats were randomly assigned into 4 groups： Control group （ administered with a single intraperitoneal injection of citric buffer）, DM group （ administered with a single intraperitoneal injection of STZ）, DMSO group （diabetic rats intraperitoneally adminis- tered with mixture of dimethylsulfoxide and normal saline, once per day） and CUR group （diabetic rats intraperitoneally administered with CUR, once per day）. Three months after diabetes onset,malondialdehyde（MDA） and reduced glutathione hormone （GSH） in retina were detected, glial fibrillary acidic protein （GFAP） in retina were re- vealed by immunohistochemistry, retinal GFAP and glutamine synthetase （GS） were ob- served by Western-blot, and glutamate in retinal were detected by high performance liq- uid chromatogram. Results Levels of retinal MDA,glutamate and GSH were（3.47 ± 0.51 ） nmol · mg^- 1 prot, （ 35.5±4.8 ） nmol · mg^- 1 and （ 98.51 ± 9.82 ） mg · g^-1 prot in control group, （ 7.21 ±0.83） nmol · mg^- 1 prot, （ 49.4 ± 4.1 ） nmol · mg^- 1 and （ 54.85 ± 3.47）mg · g^-1 prot in DM group, （7.13 ±0.74） nmol · mg^- 1prot, （52.5 ±3.7） nmol · mg^- 1 and （59.61 ±5.38）mg · g^-1 prot in DMSO group,and （3.91 ±0.43） nmol · mg^- 1 prot, （86.93 ±7.84）mg · g^-1 prot and （ 38.4 ± 3.7 ） nmol · mg^- 1 in CUR group. Compared with control group, levels of retinal MDA and glutamate were in- creased, and GSH were decreased in DM and DMSO groups （ all P 〈 0.05 ）, while, levels of retinal MDA, GSH and glutamate in CUR group showed no statistical difference compared with control group（ all P 〉 0.05 ）. Furthermore, up-regulation of ret- inal GFAP and down-regulation of retinal GS were detected in DM and DMSO group

关 键 词：糖尿病视网膜病变 姜黄素 氧化应激 MÜLLER细胞 

分 类 号：R587.2[医药卫生—内分泌]