IL-15对树突状细胞激活的T淋巴细胞免疫反应的作用观察  

Effect of IL-15 on T lymphocyte-mediated immune responses activated by dendritic cells

作　　者：赵煜[1] 张涛[1] 谭勇[1] 彭晶晶[1] 周进军[1] 许涛[1] 付曾强 

机构地区：[1]中国人民解放军成都军区总医院肿瘤中心,成都市610083

出　　处：《中国肿瘤临床》2013年第3期127-130,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:观察IL-15和IL-2对树突状细胞疫苗(dendritic cells,DC)激活的淋巴细胞免疫反应的作用。方法:取健康小鼠的脾淋巴细胞,在体外采用肿瘤抗原负载的DC疫苗与脾淋巴细胞混合后,分别加入IL-15或IL-2培养7d。采用流式细胞术检测淋巴细胞免疫表型的变化;使用酶联免疫斑点实验(ELISPOT)检测分泌干扰素(interferon,IFN)-γ的细胞数量;利用51Cr释放法检测淋巴细胞对肿瘤细胞的杀伤活性。结果:IL-15联合DC疫苗增强了T细胞反应,包括淋巴细胞免疫表型和功能的改变。结论:与IL-2相比,IL-15能更好的增强DC疫苗诱发的免疫反应,为IL-15与DC疫苗的联合应用提供了一定的依据。Objective：This study aimed to observe the ability of interleukin-15 （IL- 15 ） or interleukin-2 （IL- 2） to act as a potent adjuvant for a dendritic cell （DCs） –directed vaccine strategy to activate T-cell responses in vitro. Methods：Splenic lymphocytes from na ？ve mice were prepared. Then, bone marrow-derived DCs （BMDCs） were pulsed with tumor antigens and cultured with the splenocytes in the presence of IL-15 or IL-2 for 7 days. The activated T lymphocytes were examined by flow cytometric analysis, interferon-enzyme-linked immunospot, and cytotoxicity assays. Results：IL-15 resulted in an augmentation of T-cell responses following immunizations with BMDCs. The enhanced response was based on the phenotype characteristics of the lymphocytes and their functions. Conclusion： IL-15 may act as a potent adjuvant for the DC-directed vaccine strategy.

关 键 词：白细胞介素-15 白细胞介素-2 树突状细胞 细胞毒T淋巴细胞 

分 类 号：R392[医药卫生—免疫学]