修复脊髓损伤的组织工程多孔支架的实验研究  

Experimental Study on Tissue Engineering Scaffolds in Treatment for Spinal Cord Injury

作　　者：安晓[1] 史廷春[1] 陈美花[1] 蔡建辉[1] 岳秀艳[1] 

机构地区：[1]杭州电子科技大学生物制造研究中心,浙江杭州310037

出　　处：《航天医学与医学工程》2013年第1期56-59,共4页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助(60952001);浙江省自然科学基金资助(2009C33014);浙江省科技计划公益项目资助(Y2080884)

摘　　要：目的研究一种用于修复脊髓损伤的组织工程多孔支架。方法采用聚乳酸-基乙酸共聚物[poly(latic-co-glycolic acid),PLGA]作为脊髓支架材料,通过低温快速成形工艺和致孔剂浸出工艺,制备了孔径在300～500μm,大孔相互贯通的脊髓支架,然后将雪旺氏细胞种植在此支架材料上研究该支架的生物相容性。结果成型支架的宏观孔隙圆润、规则;包含大量无规则的微孔结构,孔隙的贯通性良好,支架平均孔隙率达89.92%;并具有较好的力学性能。生物学观察显示雪旺氏细胞在PLGA支架上生长迅速,繁殖能力强。结论该支架具有良好的生物相容性和生物活性,可以作为修复脊髓损伤的支架。Objective To investigate tissue engineering scaffolds for treatment of spinal cord injury. Methods Poly（ latic-co-glycolic acid）（PLGA） was adopted to construct spinal scaffold, and the interconnected macro- porous spinal scaffolds with pore diameter from 300 Ixm to 500 μm were prepared by low-temperature deposition manufacturing （LDM） and porogen particulate leach technology. Then biocompatibility of the scaffold was investigated through Schwann cell culture. Results The big pores of scaffold were round and regular. The scaf-fold included a large number of irregular pore structure with good penetrating property. The average porosity of scaffold was 89.92% and with good mechanical properties. Biological examinations confirm that Schwann cell growed rapidly after being inoculated in PLGA scaffolds. Conclusion The PLGA scaffolds have excellent bio-compatibility and bioactivity and can be used in treatment of spinal cord injury.

关 键 词：组织工程 支架 PLGA 低温成形技术 

分 类 号：R318.1[医药卫生—生物医学工程]