注射用尖吻蝮蛇凝血酶对兔凝血功能的影响  

Effect of hemocoagulase actutus for injection on blood coagulation function in rabbits

作　　者：樊华[1] 张鹏[2] 康强[1] 王秀英[1] 

机构地区：[1]辽宁省食品药品检验所,辽宁沈阳110023 [2]沈阳市食品药品检验所,辽宁沈阳110000

出　　处：《中国药理学与毒理学杂志》2013年第1期43-47,共5页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的研究注射用尖吻蝮蛇凝血酶(Hem)对兔凝血功能的影响,为临床应用提供实验依据。方法于日本大耳白兔耳静脉分别一次性iv给予Hem 0.25,0.5和1.0 U·kg-1和阳性对照药注射用血凝酶(HAI)1.0克氏单位(KU)·kg-1,于给药前(0 min)和给药后10 min,30 min,2 h和12 h分别采血,应用Lee-White试管法测定全血凝血时间(CT),血球计数仪测定血小板计数(PLT),C2000-4高性能血凝仪测定凝血酶原时间(PT)、凝血酶时间(TT)、血浆纤维蛋白原(FIB)和鞣花酸活化部分凝血活酶时间(APTT)。结果正常对照组不同时间点各指标均无明显变化。与给药前比较,CT在给予Hem 0.25,0.5和1.0 U·kg-1和HAI 1.0 KU·kg-1后10 min～12 h明显缩短(P<0.05);PLT在Hem 1.0 U·kg-1给药后10～30 min明显增加(P<0.05);APTT在Hem 1.0 U·kg-1(10 min～2 h)和HAI 1.0 KU·kg-1(30 min～12 h)明显降低(P<0.05);PT在Hem 0.25 U·kg-1(10 min),0.5 U·kg-1(10 min～2 h)和1.0 U·kg-1(10～30 min)及HAI 1.0 KU·kg-1(10～30 min)明显降低(P<0.05);TT在Hem 1.0 U·kg-1(10 min～12 h)和HAI 1.0 KU·kg-1(30 min)明显降低(P<0.05);FIB在Hem 0.25 U·kg-1(30 min),0.5 U·kg-1(10～30 min)和1.0 U·kg-1(10 min～2 h)及HAI 1.0 KU·kg-1(10～30 min)明显升高(P<0.05)。结论Hem 1.0 U·kg-1在给药后10 min即有促凝血作用,TT缩短可持续12 h。OBJECTIVE To investigate the effect of hemocoagulase actutus for injection （Hem） on the blood coagulation system in rabbits. METHODS The rabbits were divided into four groups. Three groups were given 0.25, 0.5 and 1.0 U· kg^-1 of Hem separately by ear intravenous injection, and one group was given hemocoagulase atrox for injection （HAl） 1.0 Klobusitzky unit （KU） · kg^-1 as positive control group. Before administration and 10 rain, 30 rain, 2 h and 12 h after administration, the coagula- tion time （CT） and platelet （PLT） were determined with Lee-White tube method and globulimeter, respectively. The prothrombin time （ PT）, thrombin time （TT）, fibrinogen （FIB） and activated partial thromboplatin （APTT） were measured by C2000-4 high performance blood coagulation analyzer. RESULTS No index at different times in normal control group had obvious change. CT was shorted 10 min-12 h after Hem 0.25, 0.5 and 1.0 U· kg^-1 and HAl 1.0 KU.kg-lwere given （P〈0.05）. PLT was increased 10 - 30 min after Hem 1.0 U · kg^-1 （ p 〈 0.05） was adminstered. APTT was declined 10 min-2 h after Hem 1.0 U· kg^-1 was given and 30 min-12 h after HAl 1.0 KU· kg^-1 （P〈0.05） was given. PT was shorted 10 min after Hem 0.25 U· kg^-1 ,10 min -2 h after Hem 0.5 U· kg^-1 , 10 -30 min after Hem 1.0 U· kg^-1 and 10-30 rain after HAl 1.0 KU· kg^-1 （P〈0.05）. TI was decreased 10 min -12 h after Hem 1.0 U· kg^-1 and 30 min after HAl 1.0 KU · kg^-1 （P 〈0. 05）. FIB was increased 30 min after Hem 0.25 U· kg^-1 ,10-30 min after Hem 0.5 U· kg^-1, 10 min-2 h after Hem 1.0 U· kg^-1 and 10-30 min after HAl 1.0 KU· kg^-1（P〈0.05）. CONCLUSION Hem 1.0 U· kg^-1 remarkably promotes blood coagulation 10 min after administration, and the decrease of TT lasts for 12 h.

关 键 词：尖吻蝮蛇凝血酶 凝血酶 凝血功能试验 

分 类 号：R96[医药卫生—药理学]