增强UV-B辐射对拟南芥叶肉细胞蛋白的影响  

Effect of Enhanced UV-B Radiation on Arabidopsis Mesophyll Cell Protein

作　　者：魏小丽[1] 郑娜[1] 李晓阳[1,2] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,临汾041004 [2]吕梁市离石区江阴高级中学,吕梁033000

出　　处：《植物研究》2013年第2期186-190,共5页Bulletin of Botanical Research

基　　金：国家自然科学基金资助项目(30671061);山西省自然科学基金资助项目(20081101)

摘　　要：采用不同剂量的UV-B辐射处理4周龄的野生型拟南芥幼苗(Columbia-0),分别采用丙酮沉淀法和TCA-丙酮法提取其叶肉细胞中的蛋白质,进而研究分析拟南芥叶肉细胞中蛋白质的含量与组成对不同强度UV-B辐射的响应。结果显示,两种方法相比较,TCA-丙酮法所提取得到的蛋白含量相对较多,更适合于分析增强UV-B辐射对拟南芥叶肉细胞蛋白质的影响;而两种方法所提取得到的蛋白质含量的变化趋势相同,随着UV-B辐射剂量的增加,蛋白质含量呈先增加后减少的趋势,B2组达到了最大。此外,蛋白条带的数目和表达量也都发生了显著变化,同样也是以中剂量处理组(B2组)变化最为明显,既有新增条带,又有消失条带。这可能是由于拟南芥在受到低剂量的UV-B辐射时,可以激活自身一些抗性基因的表达而诱导产生抗性蛋白,进而抵御UV-B的伤害;而当受到高剂量的UV-B辐射时,损伤自身的蛋白质合成途径,影响蛋白的合成。Four-week-old wild-type Arabidopsis seedlings （Columbia-O） was treated using different doses of UV- B radiation, the protein in the mesophyll cells was extracted using acetone precipitation and TCA-acetone, then Arabidopsis mesophyllcellular protein content and composition of the different intensity of UV-B radiation response were analyzed. The results showed that comparing two methods, the protein content of the TCA-acetone extract is relatively higher, which was more suitable for the analysis of enhanced UV-B radiation on Arabidopsis mesophyll cell protein; The changes of protein contents by two extraction methods showed the same trend, along with the increasing of UV-B radiation dose, the protein content increased first and then reduced, B2 group reached a maximum. In addition, the number of protein bands and expression has taken place significant change, the most obvious changes were also in the middle dose treatment group （B2 ）, both new bands and disappearance bands. This may be due to the Arabidopsis thaliana can activate some of its own resistance gene expressions and induce resistance protein by low doses of UV-B radiation, and thus resist the damage of UV-B; however, when subjected to high doses of UV-B radiation, damage their own protein synthesis pathway, and affect protein synthesis.

关 键 词：UV—B辐射 拟南芥 蛋白 

分 类 号：Q512[生物学—生物化学]