根癌农杆菌介导合欢转TaNHX2基因体系的优化  

Establishment and Optimization of Genetic Transformation and Introduction of TaNHX2 Gene by Agrobacterium tumefaciens in Albizia julibrissin

作　　者：周祥明[1,2,3] 郝志愚[1] 夏时云[1] 王姝[1] 宋建[1] 陈受宜[4] 刘仲齐[1] 

机构地区：[1]天津市农业生物技术研究中心,天津300384 [2]北京林业大学林木育种国家工程实验室,北京100083 [3]北京林业大学林木花卉遗传育种教育部重点实验室,北京100083 [4]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《植物研究》2013年第2期197-201,共5页Bulletin of Botanical Research

基　　金：北京林业大学林木育种国家工程实验室开放课题(FOP2010-13);天津市科技计划项目(12ZCDZNC04700);天津市科技计划项目(10JCYBJC09500);"十一五"国家科技支撑计划(2009BADA3B01);国际科技合作项目(2011DFA30990)

摘　　要：通过对农杆菌菌液浓度、侵染时间、预培养时间和共培养时间等影响转化效率的因素进行优化,建立了合欢农杆菌转化体系。在此基础上,利用农杆菌介导法将TaNHX2基因导入合欢基因组内,获得大量Kan抗性再生植株。常规PCR和实时荧光PCR检测结果表明,外源基因已整合到合欢基因组中,并正常转录。Some factors influencing the transformation efficiency, such as the concentration and infection duration of Agrobacterium tumefaciens, pre-culture and co-culture duration, were optimized to establish a stable Agrobacterium mediated gene transformation protocol of Albizia julibrissin Duraxx. The TaNHX2 gene was transformed into A. julibrissin. Mediated by A. tumefaciens, lots of regenerated plants resistant to Kanamycin （Kan） were obtained. PCR analysis and real time PCR detection of some transgenic plants showed that the TaNHX2 gene was evidently integrated into the genome of transgenic plants and could be transcribed properly.

关 键 词：TaNHX2 合欢 遗传转化 农杆菌 

分 类 号：S332.6[农业科学—作物遗传育种]