长白落叶松群体4CL基因单核苷酸多态性分析  

SNP Analysis of 4CL Gene in Larix olgensis Henry Populations

作　　者：徐悦丽[1] 张含国[1] 施天元[1] 姚宇[1] 张磊[1] 王艳红[1] 刘灵[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《植物研究》2013年第2期208-213,共6页Bulletin of Botanical Research

基　　金：东北林业大学创新课题"落叶松4CL基因的单核苷酸多态性分析"

摘　　要：以黑龙江省林口县青山林场21年生长白落叶松(Larix olgensis Henry)异地保存种为材料,利用SNP标记方法和DNAMAN软件分析白刀山种源子代林的单核甘酸多态性情况。从分子水平证明长白落叶松具有丰富的遗传多样性,用4CL-A引物检测到178个SNP多态位点。共测序240条EST序列,测序成功193条,测序成功率为80.42%。本研究178个SNP变异中,有126个属于转换类型,占总变异的70.79%;有52个属于颠换类型,占总变异的29.21%,变异类型符合,转换∶颠换=7∶3。在转换类型中,A/G和C/T转换分别占37.08%和33.71%;颠换类型中G/C、A/C、A/T、和G/T分别在占7.87%、8.99%、7.87%和4.99%。但每个家系内的转换和颠换的比例相差较大,没有规律。转换和颠换的比例最大的是554号家系,其比值为7∶1,最小的家系是855号家系,其比值为3∶4。With the off-site preservation forest 21-year old Larix olgensis in Qinshan forestry centre in Linkou, in Heilongjiang province as the materials, the resources of Baidaoshan were analyzed using SNP molecular markers and he software DNAMAN. There is genetic diversity in L. olgensis. SNP marker has proved the provenance of L. olgensis. Rupr has a wealth of genetic diversity from the molecular level, and the marker indicates that the genetic variation mainly exists within populations, smaller variation exists among population SNP Primers detected of 178 SNPs. It has sequenced a total of 240 EST sequencing, and the successfully are 193 EST sequencing, in which the successful percentage is 80.42%. Using the software DNAMAN analysis the sequencing result found that there are 178 SNPs, 126 belong to transition type, accounting for 70.79% of the total variance; with 52 belong to transversion type, accounting for 29.21% of the total variance, the mutant type roughly in line, transition：transversion = 7：3. In the transition type, A/G and C/T transition account for 37.08% and 33.71%, respectively; some types of transversion G/C, A/C, A/T, and G/T respectively in 7.87%, 8.99%, 7.87% and 4.99%. But within each family, transition and transversion are quite different, there is no law. The largest proportion of conversion and transversion is No. 554 family, a ratio of 7：1, the smallest pedigree is 855 pedigree with ratio 3：4.

关 键 词：长白落叶松 家系 单核甘酸多态性 4CL基因 

分 类 号：S722.5[农业科学—林木遗传育种]