四川呼吸道合胞病毒F蛋白基因序列和抗原表位变异分析  

Gene Sequence and Antigenic Epitope Variation within the F Protein of Respiratory Syncytial Virus in Sichuan,China

作　　者：张梦妍[1,2] 廖虹瑜[1] 范雪佳[1] 鲁蕾[1] 裴晓方[1] 许欣[1] 

机构地区：[1]四川大学华西公共卫生学院·华西第四医院医学检验教研室成都,610041 [2]陕西省疾病预防控制中心,西安710054

出　　处：《四川大学学报（医学版）》2013年第2期175-178,共4页Journal of Sichuan University(Medical Sciences)

基　　金：“艾滋病和病毒性肝炎等重大传染病防治科技”重大专项“传染病检测技术平台”项目;“云南及周边省市传染病病原谱流行规律研究”课题(No.2009ZX10004-212)资助

摘　　要：目的分析呼吸道合胞病毒(RSV)四川分离株F蛋白基因序列,探讨F蛋白基因和抗原表位变异情况。方法 RT-PCR方法扩增F蛋白近全长序列并测序,分析核苷酸和氨基酸变异位点,比较不同亚型和基因型间中和表位、细胞毒性T淋巴细胞(CTL)表位基因组成。结果 10株RSV F蛋白的核苷酸和氨基酸P-distance分别为0.102±0.005和0.058±0.006,中和表位47F和L4在亚型间高度保守,而RS-348和7C2在亚型内保守,CTL表位HLA B*57、HLA A*01和HLA Cw*12也是亚型内高度保守,亚型间存在个别氨基酸变异。结论 RSV四川分离株F蛋白基因保守度较高,抗原表位在亚型内保守,已在A亚型上识别的CTL表位可能在B亚型内不能识别。Objective To investigate the variation of cytotoxic T-lymphocyte （CTL） and neutralizing epitopes in F protein of respiratory syncytial virus （RSV） isolated in Sichuan. Methods Nearly full-length of F protein gene of 10 strains of RSV isolated in Sichuan was amplified by RT-PCR and sequenced. The genetic variations, especially the CTL and neutralizing antibody epitopes within different subtypes and genotypes were analyzed and compared. Results The F protein of RSV is highly conserved within the two subtypes, with the P-distances of nucleotide and amino acids were 0. 102±0. 005 and 0. 058±0. 006, respectively. Neutralizing epitopes 47F and L4 were conserved between the subtypes, but RS-348 and 7C2 were only conserved within the subtypes. CTL epitopes HLA B ＊ 57, HI.A A ＊ 01 and HLA Cw ＊12 were conserved only within subtype A. There were specific different sites between the subtypes. Conclusion The sequences of F protein from Sichuan RSV isolates were highly conserved, so as the epitopes on F-protein within subtypes, the identified CLT epitopes in subtype A may not be recognized in subtype B virus.

关 键 词：呼吸道合胞病毒 F蛋白 细胞毒性T淋巴细胞表位 中和表位 

分 类 号：R392[医药卫生—免疫学]