Anethum graveolens Linn.(dill) extract enhances the mounting frequency and level of testicular tyrosine protein phosphorylation in rats  

作　　者：Sitthichai IAMSAARD Thawatchai PRABSATTROO Wannisa SUKHORUM Supaporn MUCHIMAPURA Panee SRISAARD Nongnut UABUNDIT Wipawee THUKHAMMEE Jintanaporn WATTANATHORN 

机构地区：[1]Department of Anatomy,Faculty of Medicine,Khon Kaen University [2]Integrative Complementary Alternative Medicine Research and Development Group,Faculty of Medicine,Khon Kaen University [3]Department of Physiology,Faculty of Medicine,Khon Kaen University

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2013年第3期247-252,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project (No.53-03-2-00-008) supported by the Office of Research Administration,Khon Kaen University,Thailand

摘　　要：Objective:To investigate the effect of Anethum graveolens(AG) extracts on the mounting frequency,histology of testis and epididymis,and sperm physiology.Methods:Male rats induced by cold immobilization before treating with vehicle or AG extracts [50,150,and 450 mg/kg body weight(BW)] via gastric tube for consecutive 1,7,and 14 d were examined for mounting frequency,testicular phosphorylation level by immunoblotting,sperm concentration,sperm acrosome reaction,and histological structures of testis and epididymis,respectively.Results:AG(50 mg/kg BW) significantly increased the mounting frequency on Days 1 and 7 compared to the control group.Additionally,rat testis treated with 50 mg/kg BW AG showed high levels of phosphorylated proteins as compared with the control group.In histological analyses,AG extract did not affect the sperm concentration,acrosome reaction,and histological structures of testis and epididymis.Conclusions:AG extract enhances the aphrodisiac activity and is not harmful to sperm and male reproductive organs.Objective： To investigate the effect of Anethum graveolens （AG） extracts on the mounting frequency, histology of testis and epididymis, and sperm physiology. Methods： Male rats induced by cold immobilization before treating with vehicle or AG extracts [50, 150, and 450 mg/kg body weight （BW）] via gastric tube for consecutive 1, 7, and 14 d were examined for mounting frequency, testicular phosphorylation level by immunoblotting, sperm concentration, sperm acrosome reaction, and histological structures of testis and epididymis, respectively. Results： AG （50 mg/kg BW） significantly increased the mounting frequency on Days 1 and 7 compared to the control group. Additionally, rat testis treated with 50 mg/kg BW AG showed high levels of phosphorylated proteins as compared with the control group. In histological analyses, AG extract did not affect the sperm concentration, acrosome reaction, and histological structures of testis and epididymis. Conclusions： AG extract enhances the aphrodisiac activity and is not harmful to sperm and male reproductive organs.

关 键 词：Anethum graveolens Linn. （dill） Mounting frequency Testicular phosphorylation Sperm acrosome reaction 

分 类 号：R698.2[医药卫生—泌尿科学]