PCR和IFA测定猪圆环病毒2型半数细胞培养感染量的比较  

Comparison between PCR and indirect immunofluorescence assay(IFA) methods for determining PCV2 TCID_(50)

作　　者：王林山[1,2] 尹燕博[2] 邹勇[1] 何锡忠[1] 韩芳婷[3] 倪建平[1,4] 赵本进[1,4] 李春华[1,4] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]青岛农业大学动物科技学院,青岛266109 [3]上海师范大学生命与环境科学学院,上海200234 [4]上海佳牧生物制品有限公司,上海201106

出　　处：《上海农业学报》2013年第1期34-38,共5页Acta Agriculturae Shanghai

基　　金：上海市科委成果转化项目(113919N0900);上海市农业科学院开发基金项目(KF05)资助

摘　　要：采用聚合酶链式反应(PCR)和间接免疫荧光法(IFA)测定PCV2 SN07-1 2株的半数细胞培养感染量(TCID_(50)),初步比较两种方法测定病毒滴度的差异。参照Reed-Muench法计算TCID_(50),并采用荧光定量PCR(Real-time PCR)法对试验结果进行验证。对于同一病毒样品,IFA法测出的TCID_(50)为10^(6.5)/mL,PCR法测出的TCID_(50)为10^(5.0)/mL,结果表明:IFA法比PCR法更敏感,更适宜用于测定PCV2的效价。Both PCR and IFA methods were used to determine the TCID50 of PCV2 SN07-12 strain and the difference of the virus titers determined by both methods was preliminarily compared. Also the TCID50 was calculated according to the Reed-Muench method and the accuracy of both methods was verified by the real-time PCR method. For the same virus sample, the TCID50 determined by the IFA and PCR methods were respectively 10^6.5/mL and 10^5.0/mL, and the result showed that the IFA method was more sensitive and more suitable for determining PCV2 titer than the PCR method.

关 键 词：猪圆环病毒2型 间接免疫荧光 聚合酶链式反应 半数细胞培养感染量 

分 类 号：S852.65[农业科学—基础兽医学]