LC调节人成骨样MG-63细胞表达OPG、RANKL分子机制的研究  

Molecular mechanism of LC on regulating the expression of OPG and RANKL in human osteoblast-like MG-63 cells

作　　者：祁琳[1,2] 张鹏[3] 屈野[4] 王文良[3] 毛立群[1] 王越[1,2] 李灵芝[1,5] 

机构地区：[1]天津市职业与环境危害生物标志物重点实验室,天津300162 [2]武警后勤学院免疫学教研室 [3]武警后勤学院附属医院骨科 [4]武警后勤学院微生物学教研室 [5]武警后勤学院药物化学教研室

出　　处：《中国骨质疏松杂志》2013年第2期125-130,共6页Chinese Journal of Osteoporosis

基　　金：天津市自然科学基金重点项目(12JCZDJC26300;10JCZDJC21100;10JCYBJC10800);武警后勤学院科学技术研究重点;面上项目(WHZ201202;WY200902;WY200914)

摘　　要：目的探讨大黄酸哌嗪雌酚酮(rhein-piperizinyl-estrone,命名为LC)调节人成骨样MG-63细胞表达骨保护素(osteoprotegerin,OPG)、NF-κB激活受体配体(receptor activator of NF-κB ligand,RANKL)的分子机制。方法在原工作基础上,选择兼有两种雌激素受体(estrogen receptor,ER)亚型表达的人成骨样MG-63细胞为研究模型,采用RT-PCR、免疫印迹及小RNA干扰等技术,探讨LC对人成骨细胞产生的骨吸收调节因子OPG、RANKL表达的作用及作用机制。结果 LC可上调MG-63细胞OPG表达及下调RANKL表达,该作用可被纯ER阻断剂ICI 182,780完全阻断,应用小RNA干扰技术进一步证实LC对成骨细胞OPG、RANKL表达的调节作用主要是由ERα介导的。结论 LC调节成骨细胞表达OPG、RANKL是经ER途径、主要是由ERα介导的。Objective To investigate the molecular mechanism of rhein-piperizinyl-estrone （LC） on regulating the expression of osteoprotegerin （OPG） and receptor activator of nuclear factor-KB ligand （RANKL） in human osteoblast-like MG-63 cells. Methods Based on our previous studies, human osteoblast-like MG-63 cells, which contained two isoforms of estrogen receptors （ER）, were selected as model for this study. Effect of LC on the expression of OPG and RANKL produced in human osteoblast- derived cells and its molecular mechanism were studied using RT-PCR, Western blotting, and small interfering double-stranded RNAs （siRNA） technology. Results LC up-regulated the expression of OPG and down-regulated the expression of RANKL. This effect could be blocked by pure ER antagonist, ICI 182, 780. The effect of LC on regulating the expression of OPG and RANKL was mediated by ERα, which was further demonstrated using siRNA technology. Conclusion The effect of LC on regulating the expression of OPG and RANKL in osteoblasts is through the ER pathway, and it is mediated primarily by ERα.

关 键 词：大黄酸哌嗪雌酚酮(LC) 成骨细胞 骨保护素(OPG) NF-κB激活受体配体(RANKL) 雌激素受体(ER) 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]