丙型肝炎病毒NS5b区与5’-非编码区不同基因区RNA检测结果的比较  

Significance of comparing hepatitis C virus (HCV) RNA by polymerase chain reaction between at NS 5b region and 5’-noncoding region

作　　者：许青田[1] 季颖[2] 杜绍财[2] 王会民[1] 李新月[1] 陶其敏[2] 

机构地区：[1]郑州市第六人民医院,河南郑州450005 [2]北京医科大学人民医院肝病研究所,北京100044

出　　处：《临床肝胆病杂志》2000年第4期214-215,共2页Journal of Clinical Hepatology

摘　　要：为探讨丙型肝炎病毒 (HCV)NS 5b区与 5’ -非编码区 ( 5’ -NCR)不同基因区RNA检测结果的区别及临床应用价值。利用逆转录聚合酶链反应 (RT -PCR)技术检测 148例肝炎患者血清HCVNS 5b基因的cDNA ,并与5’ -NCR基因区cDNA检测技术进行了比较。结果　HCVNS 5b基因区RNA检出率为 2 4.3% ( 34/ 148) ,5’ -NCR基因区RNA检出率为 31.1% ( 47/ 148)。HCV 5’ -NCR基因区扩增效果较好 ,而HCVNS 5bPCR敏感性接近于HCV 5’-NCR检测结果 ,亦可直接应用于临床RNA检测。To determine the influence on the detection of HCV RNA by using different primerpairs,reverse transcription polymerase chain reaction(RT-PCR) for NS5b region and 5'-noncoding region (5'-NCR) of HCV were used in 148 cases with hepatitis.The results show that HCV RNA positive rates were 24.3% by the primers at NS5b gene,31.1% by the primers 5-NCR gene.The detection for HCV RNA at NS5b region was as sensitive as that at 5-noncoding region

关 键 词：聚合酶锭反应 RNA 丙型肝炎 HCV 5'-NCR 

分 类 号：R512.63[医药卫生—内科学]