各型病毒性肝炎TTVDNA检测结果  

Detection of TTV Incidence Among Difeerent Types of Viral Hepatitis

作　　者：王兆荃[1] 舒波[1] 李颖[1] 李智伟[1] 王静艳[1] 汪梅 洪明 金国贤 支亚庆 

机构地区：[1]中国医科大学二院 [2]盘锦油田二院,辽宁盘锦124120 [3]辽阳市传染病院 [4]空军沈阳医院 [5]本溪县第一人民医院

出　　处：《临床肝胆病杂志》2000年第4期239-241,共3页Journal of Clinical Hepatology

摘　　要：各型肝炎中及献血者中TTV合并感染情况。设计特异性引物 ,采用半套式PCR法 ,对辽沈地区 2 0例散发性非甲 -非庚型肝炎 ,2 0例慢性乙型肝炎 ,2 0例慢性丙型肝炎及 40例重型肝炎与 5 0例献血者血清进行TTVDNA检测。非甲 -非庚型肝炎血清阳性 1例 ( 5 % ) ;重型肝炎阳性 9例 ( 2 2 .5 % ) ;慢性乙肝阳性 5例 ( 2 5 % ) ;慢性丙肝阳性 3例 ( 15 % ) ;献血者未查出TTVDNA阳性者。二株TTVDNA扩增产物的核苷酸序列分析与日本Nishizama基因同原性 98%以上。本地区献血者TTVDNA感染率极低 ;重型肝炎及慢性肝炎患者TTVDNA合并感染率较高 ,无输血史的散发性非甲 -非庚型肝炎中 ,血清TTVDNA阳性率为 5 % ,较其它报道输血后非甲 -非庚型肝炎的TTV感染率为低。It investigated infectious rate of transfusion transmitted virus (TTV) among the patients with different types of viral hepatitis and blood donor.A semi nested polymerase chain reactions(nPCR) assay was usedwith specific primers from TTV genome.20 cases of sporadic NA NG hepatitis (NA NG H),40 cases of fulminate hepatitis (FH),20 case of chronic hepatitis B (CHB),20 cases of chronic hepatitis C(CHC) and 50 cases of voluntry blood donor (BD) were examined by nPCR.PCR product of two samples was sequenced with Sequencher ABI PRISM).Positive rates of TTV DNA in NA NG H,FH,CHB,CHC and BD were 5%,22.5%,25%,15% and 0%,respectively.The partial sequence of TTV isolated in Liaoning(SYS 04 & 05)shown 98% above nucleotide identity over the corresponding region of TTV in Japan by Nishizawa.The results shown that positive rates of TTV DNA were the same in FH as CHB,and a superinfection was close relation to developed into severe or persistent disease.It was sure that a blood come from some voluntry donors was safe from TTv infection,when voluntry blood donors were detected normal of all liver function.

关 键 词：输血传播病毒 病毒性肝炎 TTV DNA 半套式PCR 

分 类 号：R512.6[医药卫生—内科学]