人参皂苷Rh2对白血病HL60细胞生长的调节作用  

Effects of Ginsenoside-Rh2 on growth regulation of leukemia HL60 cells

作　　者：姚云[1] 蒋俊锋[2] 王强利[1] 赵英侠[1] 郭春荣[1] 王奕[1] 徐维蓉[1] 

机构地区：[1]上海中医药大学组织胚胎学教研室,上海201203 [2]第二军医大学基础部组织胚胎学教研室发育生物学研究中心,上海200433

出　　处：《中华中医药杂志》2013年第3期798-801,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市教委优青项目(No.P13910);上海市教委重点学科资助项目(No.J50301)~~

摘　　要：目的:观察人参皂苷Rh2对人白血病细胞株HL60增殖及凋亡的调节作用。方法:利用流式细胞术检测白血病细胞株HL60在人参皂苷Rh2处理过程中细胞增殖周期的改变及凋亡发生的情况;另一方面通过动物实验,将药物处理前后的细胞株注射裸鼠,验证该药物是否能够有效抑制HL60细胞在动物体内的恶性增殖及白血病症状。结果:药物处理后的HL60细胞在G1期比例降低,S期比例显著升高(P<0.05),药物处理后HL60细胞早期及中晚期细胞凋亡率为40%左右,明显高于未处理组HL60细胞,人参皂苷Rh2通过介导S期阻滞作用从而使HL60细胞进入凋亡程序。通过动物实验证明了人参皂苷Rh2能够抑制HL60细胞在体内的恶性增殖。结论:人参皂苷Rh2能够抑制人白血病细胞株HL60的恶性增殖并促进其发生凋亡。Objective：To explore the proliferative and apoptotic effect of Ginsenoside Rh2 on leukemia HL60 cells. Methods：We detected the apoptotic rate and proliferative cycle changes at pre-/post-administration points of Ginsenoside Rh2 using flow cytometry in HL60 cells; and we compared the organic hyperplasia and lymphocytes infiltration status in nude mice by injecting pre-/post-administrated HL60 cells, analyzed the malignancy changes between both groups to verify the possibility of Ginsenoside Rh2＇s inhibition on HL60 cells in vivo. Results：The amount of Ginsenoside Rh2-treated HL60 cells was significantly small in G1 phase and large in S phase（P〈0.05）, which leaded HL60 cells into apoptosis. We also obtained this conclusion by observing reduced malignant tendency of HL60 cells in animal experiments. Conclusion：Ginsenoside Rh2 is capable of regulating the growth of leukemia HL60 cells by inhibiting its proliferation and promoting apoptosis.

关 键 词：人参皂苷RH2 白血病 细胞增殖 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]