哺乳动物尿液中钙镁离子浓度的^1H NMR测定方法  

An NMR Method for Determining Concentrations of Calcium and Magnesium Ions in Mammalian Urines

作　　者：江李苗[1,2] 唐惠儒[1] 

机构地区：[1]中国科学院生物磁共振分析重点实验室(筹),波谱与原子分子物理国家重点实验室,武汉磁共振中心(中国科学院武汉物理与数学研究所),湖北武汉430071 [2]中国科学院大学,北京100049

出　　处：《波谱学杂志》2013年第1期80-85,共6页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金资助项目(20825520,20775086,20775087,21175149)

摘　　要：哺乳动物体液中的钙镁离子浓度能够反映体内营养代谢的平衡与一些疾病的发生发展.尿液中钙镁离子浓度的准确测量需要不受基质影响的便捷新方法.该文使用过量乙二胺四乙酸(EDTA)为探针并通过1 H NMR方法测定钙镁离子与EDTA络合物的含量,建立了一种快速原位的尿液钙镁离子浓度测量方法.将此方法分别用于人、大鼠及小鼠的尿液测定,发现3类哺乳动物的尿液钙镁离子浓度存在显著的物种依赖性和较大的相同物种个体间差异.上述为生物体液中钙镁离子浓度的快速定量测量提供了便捷方法,也提供了3类哺乳动物尿液钙镁离子浓度的基础数据.Concentration of the calcium and magnesium ions in mammalian biofluids car- ries important information on metabolic homeostasis for nutrients and pathophysiology of some diseases. Methods are in urgent need to achieve rapid quantitative measure-ments of these ions in urines with little matrix effects. In this work, we report a simple 1 H NMR method for measuring urinary concentrations of these ions by using ethylenedi-aminetetraacetic acid （EDTA） as a probe and measuring EDTA complexes of calcium and magnesium ions. This method was applied to analyze human, rat and mouse urine samples. The results indicated that urinary concentrations of calcium and magnesium i-ons had a clear species dependence and inter-individual variation within the same species. The method offered a simple and rapid way for quantitative measurements of calcium and magnesium ions in biofluids in situ and provided some useful data for urinary calcium and magnesium ions for man, rats and mice.

关 键 词：核磁共振(NMR) 钙离子 镁离子 尿液 

分 类 号：O482.53[理学—固体物理]