益气解毒方乙酸乙酯提取部位对鼻咽癌患者外周血树突状细胞生物学特性的影响  

Effects of ethyl acetate extract of Qi Boosting Toxin Resolving Formula on the biological properties of induced DCs from the peripheral mononuclear blood cells of untreated NPC patients

作　　者：胡梅[1] 田道法[2] 何迎春[2] 刘湘[2] 范婧莹[2] YANG PC 

机构地区：[1]湖南中医药大学医学院,长沙410007 [2]湖南中医药大学中西医结合学院 [3]McMaster University Canada

出　　处：《中国中西医结合耳鼻咽喉科杂志》2013年第1期44-47,共4页Chinese Journal of Otorhinolaryngology in Integrative Medicine

基　　金：国家自然科学基金(No.81072904;No.30973856;No.81273988);中国博士后基金(No.2011M501277);湖南省科技计划项目(No.2010SK3021);湖南省2011年研究生创新基金研究项目(No.CX2011B355);湖南省外国专家局引智项目(湘财社指2011[40]号);湖南省中医药科研项目(2010007;201142);湖南省中医五官科学重点学科;湖南省海外名师计划(湘教通2012-488)

摘　　要：目的察益气解毒方乙酸乙酯提取部位(ethyl acetate extract,EAE)对鼻咽癌(nasopha ryngealcarcinoma,NPC)患者外周血树突状细胞(dendritic cells,DCs)生物学特性及其功能的影响。方法初诊NPC患者10例,抽取肘静脉血,分离培养外周血单个核细胞(PBMC),用10%胎牛血清RPMI 1640培养基分别加不同浓度益气解毒方EAE进行体外培养,倒置显微镜下观察DCs的形态学特征,流式细胞仪检测DCs表面特异分子CD1a、CD86和HLA DR的表达水平,ELISA法检测培养上清液中IL-12含量。结果益气解毒方EAE能促进NPC患者PBMC来源的DCs成熟分化并高表达成熟DCs表面的特异性分子标志物(CD1a,CD86,HLA DR),其IL 12分泌水平亦随着培养天数的增加而持续升高。结论益气解毒方EAE能明显促进NPC患者外周血来源的DCs成熟分化和功能活性表达。Objective To investigate the effects of ethyl acetate extract （EAE） from Qi-Boosting Toxin-Resolving Formula （QBTRF） on the biological properties of DCs derived from the peripheral blood mononuclear blood cells （PBMC） of patients with NPC. Methods PBMC were isolated from the peripheral blood of 10 cases with untreated NPC, and cultured in vitro in RPMI-1640 media supplemented With 10% fetal bovine serum, added with different concentrations of EAE extracted from QBTRF. Then, observed were the morphological characteristics of DCs under inverted microscope, determined the expressive activities of specific surface molecules on DCs such as CD 1 a, CD86 and HLA-DR by flow cytometry and, and detected the content of IL-12 in the culturing supematant with the procedures of ELISA. Results It was confirmed that EAE extracted from QBTRF could promote maturing different/at/on of DCs derived from PBMC of NPC patients significantly, and express specific surface markers of matured DCs on cellular membrane （CDla, CD86 and HLA-DR） highly, with IL-12 content in culturing supernatant increased gradually with the extending of culture period day by day. Conclusion It is suggested that EAE extracted from QBTRF can significantly promote maturation of DCs derived from peripheral blood of patients with NPC and functioning activity expressing.

关 键 词：鼻咽癌 树突状细胞 生物学特性 益气解毒方 乙酸乙酯提取部位 

分 类 号：R285[医药卫生—中药学]