脂蛋白相关磷脂酶A2酶活性水平及其Ⅰ198T基因多态性与冠心病的相关性研究  

Associations of Ⅰ198T gene polymorphisms with plasma lipoprotein-associated phospholipase A2activity and coronary heart disease

作　　者：岳志红[1] 菅广敏[1] 贾玫[1] 李珊珊[1] 

机构地区：[1]北京大学人民医院检验科,100044

出　　处：《中华检验医学杂志》2013年第2期156-160,共5页Chinese Journal of Laboratory Medicine

摘　　要：目的探讨脂蛋白相关磷脂酶A2的酶活性水平及1198T多态性是冠心病的危险因素。方法采用病例对照研究，应用TaqMan-ARMSPCR方法，以2009年10月至2010年5月北京大学人民医院398例冠心病患者（CAD组）为病例组，与病例组年龄、性别相匹配的396例非冠心病对照组（NCAD组）为对照组，检测其PLA2G7的1198T基因多态性，并对Lp-PLA2的酶活性水平、胆固醇（CHO）、血糖（GLU）、三酰甘油（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、超敏C反应蛋白（Hs-CRP）、脂蛋白aLp（a）进行测定和调查，运用独立样本t检验、卡方检验、方差分析、logistic分析对数据进行分析。结果冠心病组的Lp-PLA2酶活性水平高于非冠心病对照组（31．5lnmol·ml^-1·min^-1〉21．31nmol·ml^-1·min^-1），差异有统计学意义（F=16．40，P〈0．05）；校正传统冠心病危险因子[CHO，TG，hs-CRP，Lp（a），GLU]后，Lp-PLA2酶活性的最高四分位数与最低四分位数相比，CAD的比值比（OR）为7．50（95％CI：2．34-24．05）；Lp-PLA2酶活性水平在基因型Ⅱ最高（22．68nmol·ml^-1·min^-1，P〈0．05），基因型rIvr的最低（11．35nmol·ml^-1·min^-1，P〈0．05），1198T点突变与冠心病的无明显关联（P〉0．05）。结论Lp-PLA2的酶活性水平在冠心病患者中显著升高，是冠心病的危险因子。1198T点突变与冠心病的无明显关联。Objective To investigate whether Ⅰ198T gene polymorphisms and Lp-PLA2 activity were the risk factors of CAD. Methods A case-control study was conducted in 398 people with coronary heart disease and 396 controls whose ages and sex were matched with coronary heart disease from Peking University People＇s Hospital in October 2009 to May 2010. The Ⅰ198T gene polymorphisms were detected by the amplification refractory mutation system （ARMS-PCR） using TaqMan probe. Lp-PLA2 activity, CHO, GLU,TG, HDL, LDL, hs-CRP, Lp （a） were investigated at the same time. The data were analyzed by Independent-samples T Test, Chi-square test, One-Way ANOVA, Binary Logistic Regression. Results Lp- PLA2 activity was significant higer in CAD group than that in the control group （31.51 nmol·ml^-1·min^-1〉 21.31 nmol·ml^-1·min^-1, F = 16. 40, P 〈 0.05 ）. Adjustment for various traditional cardiovascular risk factors, including ages, sex, CHO, TG, Hs-CRP, Lp（a） , and GLU, quartiles of Lp-PLA2 activity were associated with risk of CVD with a OR of 7.5 （95% C1：2. 34 - 24.05 ） for comparison of the top to bottom quartile. Lp-PLA2 activity was the highest （22. 68 nmol·ml^-1·min^-1, p 〈 0.05 ） in genotype Ⅱ and the lowest （11.35 nmol ·ml^-1·min^-1, P 〈 0.05） in genotype TT, the association between Ⅰ198T and coronary artery disease was not significant （ P 〉 0. 05 ）. Conclusions Lp-PLA2 activity was significantly higher in CAD group and was a risk factor for CAD. There was no significant association between Ⅰ198T polymorphism and CAD.

关 键 词：冠心病 多态性 单核苷酸 

分 类 号：R541.4[医药卫生—心血管疾病]