单次尾静脉注射法阿霉素大鼠肾病模型的建立  

Establishment of a rat model of nephrosis induced by single tail vein injection of doxorubicin

作　　者：张勇[1] 张蓓[1] 宁华英[1] 郭云良[1] 邢广群[2] 沈卫[1] 

机构地区：[1]青岛大学医学院,山东青岛266003 [2]青岛大学医学院附属医院,山东青岛266003

出　　处：《中国实验动物学报》2013年第1期1-4,107,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(编号:81072754);国家自然科学基金项目(编号:81072398);山东省科技发展计划(编号:2009GG10002044)

摘　　要：目的建立阿霉素大鼠肾病模型,并观察模型的动态变化。方法 26只Wistar雄性大鼠随机分为模型组(13只)和空白组(13只),模型组单次尾静脉注射阿霉素6.2 mg/kg,空白组注射等容积生理盐水。检测连续10周12 h尿蛋白定量、终末血生化指标,光镜、电镜下观察各组大鼠肾脏病理改变。结果模型组12 h尿蛋白定量造模后1周与空白组差异有显著性(P<0.01),第5周达到高峰;血总蛋白、白蛋白均低于空白组,甘油三酯、胆固醇、血尿素氮均高于空白组(均P<0.05),血肌酐差异无显著性(P=0.64)。肾脏病理改变:第5周为微小病变型,第10周为局灶性节段性肾小球硬化。结论单次尾静脉注射6.2 mg/kg阿霉素,可以成功建立渐进性的大鼠肾病综合征模型。Objective To establish a rat model of doxorubicin-induced nephrotic syndrome and observe its dynamic development. Methods Twenty-six male Wistar rats were randomly divided into model group and control group. The model group received a single tail vein injection of doxorubicin 6.2 mg/kg body weight. The control group was injected with isotonic saline. 12-hour urines were collected and checked for 12 weeks once a week. Finally the rats were sacrificed, the blood biochemical indexes were checked, and renal morphological changes were examined by light and electron micros- copy. Results The urinary protein excretion in the model group was significantly increased （ P 〈 0.01 ） at the 7th day and reached a peak at 5th week. The serum total protein and albumin were decreased, triglyceride, cholesterol and blood urea nitrogen were increased （ all P 〈 0. 05 ）, while serum creatinine was not significantly changed （ P = 0. 64）. Minimal change disease was developed at the 5th Week, and focal segmental glomerulosclerosis was observed at the 10th week. Conclusion A model of progressive nephritic syndrome in rats can be successfully established by a single tail vein injection of doxorubicin in a dose of 6.2 mg/kg body weight.

关 键 词：阿霉素 肾病综合征 蛋白尿 大鼠 

分 类 号：Q95-33[生物学—动物学]