星状神经节电刺激建立交感神经介导的急性房颤犬模型  

Electrical stimulation of the stellate ganglion for establishing a canine model of acute atrial fibrillation mediated by sympathetic nerve

作　　者：周祁娜[1] 姜涛[2] 季萌[1] 王欢[1] 徐晓霞[1] 杨曦[1] 张峰[1] 侯月梅[1] 

机构地区：[1]新疆医科大学第一附属医院心律失常科 [2]新疆医科大学第一附属医院实验动物科学部,乌鲁木齐830054

出　　处：《中国实验动物学报》2013年第1期5-10,I0001,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家国际科技合作专项项目(2011DFA32860);国家自然科学基金项目(30961032);新疆医学动物模型研究重点实验室开放课题(XJDX1103-2011-07)

摘　　要：目的建立交感神经张力异常介导的急性房颤动物模型的方法学。方法将16只随意来源犬分为三组:对照组(n=4),右侧星状神经节(RSG)组(n=6)和左侧星状神经节(LSG)组(n=6),测定心房和肺静脉不同部位的房颤诱发率、房颤持续时间。结果 RSG刺激显著增加右心房(RA)的房颤诱发率和持续时间(P<0.05),LSG刺激显著增加左心房(LA)、左上肺静脉(LSPV)、左下肺静脉(LIPV)的房颤诱发率和持续时间(P<0.05);与刺激时相比,RSG切除显著降低RA的房颤诱发率和持续时间(P<0.05);LSG切除显著降低LA、LSPV、LIPV的房颤诱发率和持续时间(P<0.05)。结论星状神经节电刺激同时快速心房起搏6h可成功建立交感神经介导的急性房颤犬模型,星状神经节电刺激使心房和肺静脉部位的房颤诱发率显著升高,房颤持续时间显著延长,去星状神经节支配可减少房颤的发生和维持。Objective To develop the methodology of establishing animal model of acute atrial fibrillation （AF） induced by increased sympathetic nerve activity. Methods Sixteen mongrel dogs weighing 18 - 25 kg were divided into 3 groups： Control group （ n = 4） underwent 6-hour rapid atrial pacing only; Right stellate ganglion （RSG） group （ n = 6） and left stellate ganglion （LSG） group （ n = 6）. AF induction rate and AF duration in the left atrium （LA） , right atrium （RA） , left superior pulmonary vein （LSPV） and left inferior pulmonary vein （LIPV） were measured. Results （ 1 ） The effect on AF inducibility： In the RSG group, the induction rate of AF was significantly increased in the RA （ P 〈 0. 05 ） compared with the baseline. However, there were no significant changes in the LA, LSPV and LIPV. In the LSG group, the induction rate of AF was significantly increased （P 〈 O. 05） , compared with the baseline in the LA, LSPV and LIPV, respectively. Compared with RSG stimulation, right stellate ganglionectomy markedly decreased the AF induction rate of RA （P 〈 0.05）. Compared with LSG stimulation, left stellate ganglionectomy markly decreased the AF induction rate of LA, LSPV and LIPV （P 〈 O. 05）. （2） The effect on AF duration： In the RSG group, the duration of AF was significantly prolonged in RA （P 〈 0.05） , compared with the baseline. In the LSG group, the duration of AF was significantly prolonged （ P 〈 0. 05 ）, compared with the baseline in the LA, LSPV and LIPV, respectively. Compared with RSG stimulation, right stellate ganglionectomy markedly shortened AF duration of RA （P 〈 0.05）. Compared with LSG stimulation, left stellate ganglionectomy markedly shortened AF duration of the LA, LSPV and LIPV （P 〈 0.05）. Conclusions Elec- trical stimulation of unilateral stellate ganglion plus rapid atrial pacing for 6 hours can successfully establish a canine model of acute AF mediated by sympathetic nerve. Stellate ganglion stimulatio

关 键 词：心房颤动 交感神经 模型 星状神经节 

分 类 号：Q95-33[生物学—动物学] R541.75[医药卫生—心血管疾病]