地塞米松抑制卡氏肺孢子菌肺炎β2-防御素和Dectin-1受体的表达  

Expression of dectin-1 and β2-defensin in rat with Pneumocystis carinii pneumonia

作　　者：郑玉强[1] 刘爱波[1] 蒲瑜[1] 蔡辉[2] 叶彬[2] 

机构地区：[1]重庆医科大学附属儿童医院临检中心,儿童发育疾病研究教育部重点实验室,儿科学重庆市重点实验室,重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆400014 [2]重庆医科大学病原生物教研室,重庆400016

出　　处：《中国实验动物学报》2013年第1期18-21,I0002,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学青年基金(81000745);重庆市卫生局医学科学技术研究项目(渝卫科教[2009]66号和(渝卫科教[2010]51号)

摘　　要：目的研究卡氏肺孢子菌肺炎(Pneumocystis carinii pneumonia,PCP)鼠肺Dectin-1和β2-防御素的表达变化,探讨地塞米松对Dectin-1和β2-防御素的影响与疾病发生的相互关系。方法实验分4组:正常对照组、Pc刺激组、PCP模型组以及PCP模型恢复组。免疫抑制方法建立PCP动物模型,改良四胺银(Grocoti's methe-namine-silver nitrate method,GMS)染色检测Pc包囊;肺组织切片HE染色观察肺组织病理变化;实时荧光定量和Western blot检测Dectin-1和β2-防御素的mRNA以及蛋白的表达。结果 Pc刺激组的Dectin-1和β2-防御素的mRNA以及蛋白的表达明显高于正常对照组(P<0.05);Pc刺激组和PCP恢复组的Dectin-1和β2-防御素的mR-NA以及蛋白显著高于PCP组(P<0.05),而PCP恢复组与PCP组肺部炎症无明显差别。结论对免疫功能正常宿主,Dectin-1受体和β2-防御素可能在防Pc感染中起重要作用;地塞米松抑制了鼠肺Dectin-1和β2-防御素的表达,这可能与PCP疾病的发生和发展有关。Objective To study the expression of dectin-1 and β2-defensin in rats with Pneumocystis carinii pneumonia （PCP） , and to explore the influence of dexamethasone on the expression of dectin-1 and β2-defensin and its relationship with progression of PCP. Methods Fifty-three rats were divided into four groups： normal control, P. carinii （Pc） stimulation, PCP model and recovered PCP model. PCP rats were induced with dexamethasone in this study, and Pc cysts were detected by Grocoti＇s methenamine-silver nitrate （GMS） staining. Real-time PCR and Western blot were used to detect the expression of deetin-I and β2-defensin in the lung tissues, and HE staining for pathological examination of the lung tissues. Results The expressions of dectin-1 and β2-defensin were significantly higher in the Pc stimulation group than that of the normal group （ P 〈 0. 05） , and in the Pc stimulation group and recovered PCP group than that of PCP model group （P 〈0. 05）. The burden of Pc cysts in recovered PCP group was less than that in the PCP group, while the inflammation was similar between them. Conclusions During the Pe infection, the expression of dectin-1 and β2-defensin in the lung of immunocompetent rats is strikingly increased, which may play an important role in the defense against the Pc inva- sion. Dexamethasone inhibites the expression of dectin-1 and β2-defensin in the rat lung, and it may be one of mechanisms of PCP development induced by P. carinii.

关 键 词：地塞米松 卡氏肺孢子菌 Dectin-1受体 β2-防御素 大鼠模型 

分 类 号：Q95-33[生物学—动物学] R379.9[医药卫生—病原生物学]