急性胰腺炎血中抗炎症细胞因子的意义  

THE ALTERATION OF ANTI-INFLAMMATORY CYTOKINE DURING ACUTE PANCREATITIS

作　　者：陈晓理[1] 黄兴兰[2] 吴浩[2] 吴晓娟[1] 汤宇[1] 

机构地区：[1]华西医科大学附属第一医院普外科,成都610041 [2]华西医科大学附属第一医院消化内科

出　　处：《中国普外基础与临床杂志》2000年第6期354-356,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的　探讨急性胰腺炎时血中抗炎症细胞因子白介素 10 (IL 10 )和转化细胞生长因子 β(TGF β)的变化及其意义。方法　实验用SD大鼠 ,分为正常对照组 (n=6 )和胰腺炎组 (n=2 0 ) ,后者采用开腹胰管注射 5 %牛磺胆酸钠(1.0ml/kg)诱导急性胰腺炎。分别于术后 2小时 (n=6 )、6小时 ((n=6 )和 2 4小时 (n=8)处死动物 ,抽血用酶联免疫吸附法 (ELISA)检测血中IL 10和TGF β及胰淀粉酶和胰腺湿重。 结果　对照组动物血IL 10为 32 .0 5± 14 .87pg/ml;TGF β为6 6 .40± 13 .2 0 pg/ml。胰腺炎后血IL 10和TGF β均升高 ,IL 10在 2、6、2 4小时时段分别为 36 .2 5± 9.76pg/ml、37.75± 6 .5 4pg/ml和 6 8.13± 19.90 pg/ml;TGF β在 2、6、2 4小时分别为 6 4.5 8± 10 .5 6pg/ml、72 .87± 18.34pg/ml和 10 3 .77± 2 8.95pg/ml。胰腺炎后 2 4小时血IL 10和TGF β与正常组相比差异有显著性意义 (P <0 .0 5 )。 结论　急性胰腺炎时血中抗炎症细胞因子升高 ,并可能和胰腺炎后期的免疫抑制效应有关。Objective To observe the alteration of anti-inflammatory cytokines (IL-10 and TGF-β) in acute pancreatitis. [WT5”HZ]Methods SD male rats were divided into 2 groups: group 1, the normal rats as a control (n=6); group 2, the acute pancreatitis induced by intraductal injection of 5% sodium cholate sulfur with the volume of 1.0 ml/kg。 The animals were killed at 2(n=6), 6(n=6) and 24 hours (n=8) after operation, the blood samples were taken for measurement of IL-10, TGF-β (by ELISA). The weight of pancreatic tissue and amylase were also observed. Results Serum IL-10 and TGF-β in control group were 32.05±14.87 pg/ml and 66.40±13.20 pg/ml, respectively. Serum IL-10 in group 2 was 36.52±9.76 pg/ml (2 hour), 37.75±6.54 pg/ml (6 hour), and 68.13±19.90 pg/ml (24 hour), respectively. Serum TGF-β in group 2 was 64.58± 10.56 pg/ml (2 hour), 72.87±18.34 pg/ml (6 hour), 103.77±28.95 pg/ml (24 hour), respectively. Compared to that of normal rats, the serum level of IL-10 and TGF-β in 24 hours of acute pancreatitis increased significantly (P<0.05). Conclusion Anti-inflammatory cytokines, both IL-10 and TGF-β were increased remarkablly in acute pancreatitis. This result indicates that there is a potential tendency of compensatory anti-inflammatory response sydrome in acute pancreatitis.

关 键 词：急性胰腺炎 细胞因子 白介素-10 转化生长因子Β 

分 类 号：R657.510.2[医药卫生—外科学]