采用冰冻切片和冷场发射电镜构建APA微囊的膜厚度及膜表面测定的方法研究  

Construction of a visual determination method on the thickness and surface of APA Microcapsules by optica microscopy with frozen section and the ice emission scanning electron microscopy

作　　者：傅红兴[1] 李慧[1] 朱雁林[1] 赵应征[1] 吴岚岚[1] 江玲[1] 

机构地区：[1]温州医学院药学院,浙江温州325035

出　　处：《温州医学院学报》2013年第2期90-92,共3页Journal of Wenzhou Medical College

基　　金：国家自然科学基金资助项目(81071277);浙江省自然科学基金资助项目(Y2080915);国家级大学生创新创业训练项目

摘　　要：目的:建立一种APA微囊的膜厚度以及表面结构的检测方法,并测定微囊在湿状态的膜厚度和干燥后的膜表面结构。方法:采用亚甲蓝染色、冰冻切片结合光学显微镜观察微囊的膜厚度,并将囊膜在37℃孵育30 d后再次观察厚度的变化;对微囊进行冷冻干燥和冷场发射观察囊膜表面和断面结构。结果:APA-Ca和APA-Ba微囊的囊膜厚度并不均匀,孵育1个月后囊膜厚度变化差异无统计学意义(P>0.05);冷场发射扫描电镜下显示,两种微囊表面均光滑、致密;冻干后APA-Ca囊膜厚度达450～690 nm,而APA-Ba的囊膜厚度约为350 nm。结论:建立了一种简单、可靠的微囊膜厚度和表面检测方法,为进一步研究APA微囊的质量奠定了基础。Objective： To explore a visual methold of the membrane thickness of APA microcapsules andtheir surface structures, and determinate the film thickness and surface characteristics. Methods： We use methylene blue to dye the film of microcapsules, then within frozen section and optical microscope the the thickness ofmicrocapsule were observed; and the thickness of microcapsules hatched in 37 ℃ for a month were also assayed. The surface of membrane were studied by ice emission scanning electron microscopy after lyophilization. Results：The member thickness of each APA microencapsules is not uniform. After incubated for 30 d in 37 ℃, there is no obviously difference in the thichness than befor （P〉0.05）. Surfaces of two microencapsules are smooth andcompact under the scanning electron microscopy. The thickness of APA-Ca microcapsules are 450-690 nm, which of APA-Ba are about 350 nm. Conclusion： The essay provided a simple method on how to determine themembrane thickness and surface, which would be an important reference to expound the APA microcapsules.

关 键 词：海藻酸钠 聚左赖氨酸 微囊 冰冻切片 冷场发射扫描电镜 

分 类 号：R944[医药卫生—药剂学]