环氧化酶-2在大鼠动脉血栓中的表达及意义  

Expression of COX-2 and its role in rat arterial thrombosis

作　　者：陈晓南[1] 曹久妹[1] 王红艳[1] 王毅盟[1] 陆林[2] 吴方[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院老年病科,上海200025 [2]上海交通大学医学院附属瑞金医院心血管科,上海200025

出　　处：《诊断学理论与实践》2012年第6期600-604,共5页Journal of Diagnostics Concepts & Practice

基　　金：上海市教委基金(11YZ57)

摘　　要：目的:探讨环氧化酶-2(COX-2)在大鼠动脉血栓中的表达及意义。方法:利用氯化铁溶液外敷大鼠左颈总动脉形成动脉血栓,HE染色后观察不同外敷时间组(1 d、3 d、7 d、14 d)大鼠的左颈总动脉血栓镜下的结构特点,采用免疫组化染色分析血栓中COX-2蛋白的表达情况,用蛋白免疫法分析不同时间组大鼠动脉血栓及循环血白细胞总蛋白,观察不同时间组动脉血栓标本及白细胞中COX-2的表达情况。结果:与正常对照组比较,外敷氯化铁溶液后,实验组大鼠的动脉可见明显的血栓形成。免疫组化染色显示,对照组未中见COX-2蛋白阳性表达,而实验组中14 d组血栓中可见多处COX-2蛋白阳性染色。蛋白印迹检测显示,对照组大鼠的COX-2仅有极少量表达,但均明显低于实验组(P<0.01),而实验组造模1 d后血栓中COX-2的表达量有所升高,3 d后表达量出现明显上升,7 d后表达量继续升高,造模14 d后,其含量进一步升高。血栓内的炎性细胞主要成分在第1天和第3天以中性粒细胞为主,而第7天和第14天则以单核巨噬细胞为主。结论:COX-2蛋白表达在大鼠颈动脉血栓中明显增加,提示COX-2的表达与动脉血栓形成有关,可能参与了动脉血栓形成的进程。Objective Inflammatory ehemokines are. involved in the pathogenesis and prognosis of arterial thrombosis. This study was to investigate the expression of eyclooxygenase-2 （COX-2） in arterial thrombosis and its significance. Methods Rat arterial thrombosis model was induced by FeC13 injury of the left carotid artery. Histological analysis of injured carotid arteries was performed by HE staining. Rats were sacrificed on day 1, day 3, day 7 and day 14 after FeCI3 injury, respectively. The expression of COX-2 protein in arterial thrombosis was assessed by immonohistochemistry （IHC）. Expressions of COX-2 protein in arterial thrombosis and circulating white blood cells at different time were measured by Western blot assay. Results FeC13 injury resulted in obvious mural thrombosis. IHC showed multiple sites of positive COX-2 staining in injured day 14 rats, while no positive staining was found in conlrols. Western blot analysis showed that COX-2 was expressed in minor degree in control group, but was high in the injured group, starting from day 1 and increased continuously to day 3, day 7 and day 14. The inflammatory cells in arte6al thrombosis were neutrophils at day 1 and day 3, and were monocytic macrophage at day 7 and day 14. Conclusions COX-2 expression increases significantly in carotid artery thrombosis and the expression of COX-2 is related to arterial thrombosis, which indicates that COX-2 might be involved in the process of arterial thrombosis.

关 键 词：环氧化酶-2 动脉血栓 大鼠 

分 类 号：R543.31[医药卫生—心血管疾病]