Effect of a Bone Graft Substitute β Tricalcium Phosphate on Osteoblastic Genes mRNA Exprssion  

作　　者：邱彤 

机构地区：[1]Biomedical Materials and Engineering Center,Wuhan University of Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2012年第5期911-915,共5页武汉理工大学学报（材料科学英文版）

基　　金：National Natural Science Foundation of China(No.81190133);Self-Determined and Innovation Research Funds of WUT(No.2012-IV-069)

摘　　要：To investigate the molecular aspects of osteoblastic interactions with β tricalcium phosphate （β-TCP） particles, human osteoblast-like MG-63 cells were cultured with β-TCP particles at a density of 6 mg/mL culture medium for 48 h. Then, the mRNA expression of selected genes were quantified by real-time polymerase chain reaction （PCR）, including the attachment-related genes （α integrin and actin）, the proliferation-related gene （c-jun）, and the osteoblastic markers genes （type I collagen, osteonectin, alkaline phosphatase, RUNX2 and osteoclain）. The results showed that β-TCP particles （the average size 809 nm） significantly promote the attachment and the proliferation of MG-63 cells, and slightly enhance the osteoblastic differentiation based on the analyses of the related genes expression. This study provided scientific evidences to better reveal the underlines of functions of β-TCP in bone repair.To investigate the molecular aspects of osteoblastic interactions with β tricalcium phosphate （β-TCP） particles, human osteoblast-like MG-63 cells were cultured with β-TCP particles at a density of 6 mg/mL culture medium for 48 h. Then, the mRNA expression of selected genes were quantified by real-time polymerase chain reaction （PCR）, including the attachment-related genes （α integrin and actin）, the proliferation-related gene （c-jun）, and the osteoblastic markers genes （type I collagen, osteonectin, alkaline phosphatase, RUNX2 and osteoclain）. The results showed that β-TCP particles （the average size 809 nm） significantly promote the attachment and the proliferation of MG-63 cells, and slightly enhance the osteoblastic differentiation based on the analyses of the related genes expression. This study provided scientific evidences to better reveal the underlines of functions of β-TCP in bone repair.

关 键 词：β-TCP osteoblastic genes mRNA expression particle size real time PCR 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]