大负荷运动及恢复期大鼠心肌超微结构及心肌营养素-1的变化  

The Changes of Cardiac Muscle Ultrastructure and Cardiotrophin-1 during Heavy Load Exercise and Recovery in Rats

作　　者：潘同斌[1] 王雅一[1] 左伟[1] 唐芳[1] 

机构地区：[1]扬州大学体育学院,江苏扬州225002

出　　处：《北京体育大学学报》2012年第12期53-56,61,共5页Journal of Beijing Sport University

基　　金：江苏省高校自然科学基础研究面上项目(项目批准号:07KJD180242)

摘　　要：目的:研究短期力竭运动及其恢复期间大鼠心肌超微结构及心肌营养素-1的变化。方法:8周龄健康雄性大鼠36只为研究对象,将其随即分为安静对照组(C)、力竭运动后即刻(Exhaustive,E0)、后12 h组(E12)、后24 h组(E24)、后48 h组(E48)、后72 h(E72)6组,每组6只。各运动组分别进行为期一周的力竭运动,安静对照组和力竭运动组分别于安静状态和末次力竭运动后不同时间点宰杀,迅速采取心肌样本,电镜观察大鼠心肌超微结构的变化,采用ELISA酶联免疫吸附反应法检测大鼠心肌营养素-1。结果:力竭运动后及恢复期心肌超微形态结构发生了不同程度的改变,其中以E0组和E24组较明显,心肌肌原纤维紊乱、扭曲,局部线粒体数量减少,甚至出现膨胀和变形。另一方面,大鼠在进行一周的大负荷运动后,心肌中的心肌营养素-1含量发生变化,力竭运动组(E0、E12、E24、E48、E72)与安静对照组相比都有显著性升高(P<0.01);其中,心肌营养素-1在E0组表达最显著,E72组有所回落(P<0.01)。结论:大负荷运动后及恢复期间,大鼠心肌内部结构遭到一定程度的破坏,同时大鼠心肌中的心肌营养素-1含量明显提高,且在E0组表达最高,提示大鼠心肌营养素-1在心肌损伤的修复中可能发挥重要作用。Objective： The purpose of this paper was to study the changes of cardiac muscle uhrastrueture and to ex- plore the changes of cardiotrophin - 1 during heavy load exercise and recovery in rat. Methods ： 36 male SD rats of 8 weeks old were randomly divided in 6 groups, and each group had six rats. Control group （ C ） and after exhaus- tive exercise group （Exhaustive,E0）, 12 h group （El2）, 24 h group （E24）, 48 h group （FA8）, 72 h group （E72）. The rats of exercise groups carried on exhaustive loaded swimming every day for one week. After the last exhaustive exercise at different time points, each rat was anesthetized intraperitoneally to slaughter, and cardiac muscles were taken. A part of cardiac muscle were fixed for producing ultrathin sections and observed to the ultra- structural changes by transmission electron microscope. The other part was to measure cardiotrophin - 1 by Elisa. Result ： After exhaustive exercise, cardiac muscle ultrastruetures had different changes in E0 and E24. The myofi- bril disorder and distorted in a range. Some mitochondrias were reduced, swollen and deformed. On the other hand, the activity of CT- 1 in all exhaustive exercise groups （E0, El2, E24, E48, E72） had a significant im- provement compared with the control group（P 〈 0.01 ）. Especially, the cardiotrophin -1 （CT- 1 ） of the cardiac muscle clearly expressed to a peak in E0, then reduced significantly in E72 （P 〈 0.01 ）. Conclusion： The ultra- structure of cardiac muscle structure in exhaustive exercise groups were significantly damaged during heavy load exercise and recovery. At same time, the eardiotrophin - 1 （ CT - 1 ） of cardiac muscle was increased during heavy load exercise and recovery. CT - 1 expressed to a peak at E0,and it indicated that cardiotrophin - 1 can be an important role to accelerate the repair of cardiac muscle injury.

关 键 词：大负荷运动 心肌超微结构 心肌营养素-1 心肌形态 

分 类 号：G804.3[文化科学—运动人体科学]