少毛北前胡水提取物镇咳作用的组效关系  

Composition activity relationship for the antitussive effect of Peucedanum harrysmithii var.subglabrum

作　　者：梁建娣[1] 封德梅 胡芳[1] 王珍[1] 梁瑾[1] 党子龙[1] 李文[1] 封士兰[1] 

机构地区：[1]兰州大学药学院,甘肃兰州730000 [2]甘肃省第二人民医院药剂科,甘肃兰州730030

出　　处：《兰州大学学报（医学版）》2012年第4期15-18,共4页Journal of Lanzhou University（Medical Sciences）

基　　金：甘肃省科技计划项目(1011WCGA162);兰州大学中央高校基本科研业务费专项资金(lzujbky-2010-191);兰州市科技计划项目(2011-1-133);教育部"春晖计划"合作科研项目(Z2009-1-62002);兰州大学国家大学生创新性实验计划(101073039)

摘　　要：目的研究少毛北前胡(PHS)水提取部位HPLC指纹图谱与其镇咳作用之间的相互关系,表征PHS水提取部位有效成分的镇咳药效物质基础。方法依次用氯仿、乙酸乙酯、乙醇、水作为提取溶剂提取PHS,制备不同提取部位样品,对这些样品进行小鼠镇咳实验,比较各提取物镇咳活性,并建立相应的HPLC指纹图谱。用灰关联度分析方法研究药效指标与HPLC指纹图谱相关信息之间的关系。结果 PHS水提取部位的镇咳活性最好,建立了PHS水提取部位HPLC指纹图谱各特征峰的镇咳谱效关系,确定各成分对镇咳作用贡献的大小顺序为特征峰编号10>5>7>1>8>9>2>4>3>6。结论 PHS水提取部位具有较强的镇咳作用,是其所有化学成分共同作用的结果 。Objective To investigate the relationship between HPLC fingerprint of the water extract part of Peucedanum harrysmithii var.subglabrum（PHS）and its antitussive action and to characterize the material basis of antitussive effect for its active ingredients.Methods Ethyl acetate,ethanol and water as the extraction solvent were used in turn to extract PHS for preparing the samples of different extract parts.The antitussive experiment of the samples from different extract parts in mice was conducted to compare their different antitussive activity and to establish the correspondent HPLC fingerprint.And the grey relational analysis was used to study the spectrum-effect relationship.Results The water extract part of PHS had better antitussive action.The relationship between the HPLC fingerprint of the water extract part of PHS and its antitussive action was established.And the contribution order of antitussive action was（number of peaks）10571892436.Conclusion The water extract part of PHS resulting from its chemical compositions together,has strong antitussive action.

关 键 词：少毛北前胡 指纹图谱 镇咳作用 灰关联度 谱效关系 

分 类 号：R917[医药卫生—药物分析学]