海岛棉DELLA蛋白基因GbGAI2的克隆及其在胚珠中表达分析  

Cloning and Expression Analysis of GbGAI2 Gene of Gossypium barbadense L.

作　　者：詹杰鹏[1] 崔百明[1] 彭明[2] 曲廷云[1] 郑银英[1] 

机构地区：[1]石河子大学生命科学学院/农业生物技术重点实验室,石河子832003 [2]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《石河子大学学报（自然科学版）》2012年第5期529-534,共6页Journal of Shihezi University(Natural Science)

基　　金：转基因生物新品种培育重大专项(2011ZX08005-002;200908005-027B)

摘　　要：赤霉素(Gibberellins,GA)在棉花纤维的发育过程中起重要作用,DELLA蛋白是GA信号通路中的起关键作用的负调控因子。本研究采用同源克隆方法,获得了海岛棉的DELLA蛋白同源基因GbGAI2。序列比对分析结果表明,GbGAI2编码的氨基酸序列具有DELLA蛋白的典型结构域,并与陆地棉DELLA蛋白GhGAI2氨基酸有90.13%相同。半定量RT-PCR分析结果表明,GbGAI2在1-5DPA和23DPA棉胚中的表达量较高,说明该基因可能在海岛棉棉纤维发育的起始阶段和棉纤维次生壁加厚阶段起作用。Gibberellins （GAs） play an important role in cotton fiber development. DELLA protein is an important negative regu- lator in GA signal transduction pathway. In this study, GbGAI2A, a homologous gene of DELLA protein, was obtained by ho- mology cloning method from Gossypium bctrbadense L.. Sequence alignment analysis showed that the deduced amino acid se- quence of GbGAI2 had all of the typical domains of DELLA proteins and displayed 90.13~ identity with that of Gossypium hirsutum L.. Semi-quantitative RT-PCR analysis showed that, in ovules,GbGAI2 transcription level was higher in 1-5 DPA and 23 DPA than other developmental stages,suggesting that GbGAI2 may play a role in the initial stage and secondary wall thick- ening stage of cotton fiber in Gossypium barbadense L.

关 键 词：海岛棉 赤霉素 DELLA蛋白 GbGAI2 

分 类 号：S562.03[农业科学—作物学] Q78[生物学—分子生物学]