理囊散中水溶性成分的超声提取及促孕活性研究  

Extraction and Fertility-Promoting Activity of Water-Soluble Components from Traditional Chinese Medicine Compound Linangsan

作　　者：曾云军[1] 李炳奇[1] 陈韩英[2] 陈晴晴[1] 谷新利[3] 

机构地区：[1]石河子大学化学化工学院/新疆兵团化工绿色过程重点实验室/省部共建国家重点实验室培育基地,石河子832003 [2]石河子大学药学院,石河子832003 [3]石河子大学动物科技学院,石河子832003

出　　处：《石河子大学学报（自然科学版）》2012年第5期598-602,共5页Journal of Shihezi University(Natural Science)

基　　金：新疆兵团重点领域科技公关项目(2011BA029);石河子大学高层次人才科研启动资金项目(RCGB201104)

摘　　要：为了研究中药复方理囊散中水溶性成分的提取工艺及其促孕活性,采用超声辅助提取法,以干膏得率为评价指标,通过单因素、正交实验优化水溶性成分的提取工艺,并考察了水溶性成分对雌性小鼠子宫系数和卵巢卵泡发育的影响。结果显示:温度为55℃,料液比为1∶12,提取3次,每次25min为复方理囊散中水溶性成分的最佳提取条件,水溶性成分高剂量组对小鼠子宫系数的影响显著高于空白组(P<0.05),水溶性成分组大型卵泡的百分比均高于空白组。结论:该工艺简单可行,理囊散中水溶性成分具有一定的促孕活性。Objective：To study the extraction technology and fertility-promoting activity of water-soluble components frorn the traditional chinese medicine compound Linangsan. Methods：The extraction rate in the extract was taken as evaluation indexes to determine the optimum extraction technology of water-soluble components. The ultrasound-assisted extraction method was a- dopted in the study, and the optimum parameters of extraction were gained by single factor and orthogonal design. The influence of water-soluble components on 21-days female mice＇s uterus coefficient and developing the follicles ovaries was investigated. Results：The optimal extraction method was 55 ~C, 25 min, 12-fold water, 3 times. The effects of water-soluble components for increasing mouse uterus coefficient is significantly higher than the blank group （P%0.05）. Conclusion： The optimal extaction technology was reasonable, water-soluble components have a certain fertility-promoting activity.

关 键 词：理囊散 水溶性成分 促孕活性 正交试验 

分 类 号：S853.74[农业科学—临床兽医学]