24个桉树品系遭受桉树枝瘿姬小蜂危害后防御酶活性变化  

Defense enzyme activity of 24 Eucalyptus varieties jeopardized by Leptocybe invasa Fisher & La Salle

作　　者：王伟[1] 徐建民[1] 李光友[1] 韩超[1] 吴世军[1] 陆钊华[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520

出　　处：《中南林业科技大学学报》2012年第6期24-28,共5页Journal of Central South University of Forestry & Technology

基　　金：国家科技支撑计划项目"高产;抗逆桉树新品种选育"(2012BAD01B0401);中央级公益性科研院所基本科研业务费专项资金项目(RITFYWZX200904);广东省林业科技创新专项资金项目"桉树良种选育和高效栽培技术研究与示范"(2009KJCX004-02)

摘　　要：选取对桉树枝瘿姬小蜂不同抗性的24个桉树品系,对其遭受危害过程中超氧化物歧化酶(SOD)、过氧化物酶(POD)和多酚氧化酶(PPO)活性变化进行测定。结果表明:受害前,桉树叶片内SOD活性、POD活性和PPO活性均表现为高抗品系>中抗品系>高感品系;在遭受桉树枝瘿姬小蜂危害后,叶片内SOD活性和PPO活性表现为先升高后下降的趋势;升高增量排名前三的品系是:柠檬桉、粗皮桉和Et12;活性下降排名前三的是Et09、DH32-29和KX12;POD活性表现为持续升高并趋于稳定的趋势,升高增量排名前三的是A107、T121和DH42-6;方差分析表明:桉树叶片内防御酶活性的变化在不同抗性品系间差异显著,表现为高抗品系>中抗品系>高感品系。24 different resistant Leptocybe invasa Eucalyptus varieties were selected to study the effects of Leptocybe invasa hazards on the varieties activites of superxide dismutase, peroxidase and polyphenol oxidase. The results show the variation tendency of SOD, POD and PPO was a descending order： ＇high-resistant varieties, middle-resistant ones and high-sensitive ones before varieties were determined by Leptocybe invasa Fisher＆ La Salle. The activities of SOD and PPO in all varieties initially increased then declined when the varieties were invaded by Leptocybe invasa. The top three varieties of incremental increase were E. Citriodora, E.pellita and ET12. The top three which declines are Et09, DH32-29 and KX12. The activities of POD in all varieties initially increased to stabilize. The top three varieties of incremental decrease were A107, T121 and DH42-6.Varianee analysis shows that there existed a huge difference of activities of defense enzymes＇ variation between the high-resistant and high-sensitive. Activities of defense enzymes can be used as a biological and chemical target to assess the resistance of Eucalyptus to Leptocybe invasa.

关 键 词：桉树品系 桉树枝瘿姬小蜂 超氧化物歧化酶 过氧化物酶 多酚氧化酶 

分 类 号：S763.43[农业科学—森林保护学]