樟树对土壤中PAHs的修复及其影响因子  

Phytoremediation of polycyclic aromatic hydrocarbons in soils by Cinnamomum camphora and impact factors

作　　者：梁小翠[1,2] 闫文德[1,3,2] 田大伦[1,3] 朱凡[1,3] 王光军[1,2] 郑威[1,4] 

机构地区：[1]中南林业科技大学,湖南长沙410004 [2]城市森林生态湖南省重点实验室,湖南长沙410004 [3]南方林业生态应用技术国家工程实验室,湖南长沙410004 [4]国家野外科学观测研究站,湖南会同418307

出　　处：《中南林业科技大学学报》2012年第12期176-180,共5页Journal of Central South University of Forestry & Technology

基　　金：国家自然科学基金项目(31070410;30571487;30771700;30870455);教育部新世纪优秀人才支持计划(NCET-10-0151);长沙市科技局项目(K1003009-61);中南林业科技大学青年科学研究基金重点项目(QJ2010008A)资助

摘　　要：采用室内盆栽实验,利用柴油设置2 g·kg-1(L1),10 g·kg-1(L2)和50 g·kg-1(L3)模拟PAHs浓度梯度,对盆栽的1年生樟树幼苗对PAHs的修复机制及影响因子进行研究。结果表明:(1)不同浓度PAHs污染(L1、L2、L3)水平下,樟树幼苗土壤中PAHs的去除率分别为85.86%、91.95%、80.80%,均高于无植物处理。(2)PAHs在樟树不同器官中的积累浓度不同。中、低浓度污染处理水平(L2、L1)下,樟树各器官对PAHs的积累作用均表现为:叶>根>干;高浓度污染处理水平(L3)下则表现为根>叶>干。不同污染处理(L1、L2、L3)水平下,樟树吸收累积作用修复PAHs污染土壤的贡献率分别为64.26%、22.81%、11.39%。(3)随着PAHs污染浓度的增大,樟树不同部位生物浓缩系数逐渐减小。叶和根是樟树富集PAHs的主要部位。(4)5cm土壤温度和多酚氧化酶是影响樟树修复PAHs污染土壤的关键因子,真菌、放线菌数量和磷酸酶活性可以作为土壤PAHs污染评价的生物指标。研究结果可为南方城市PAHs污染修复优良绿化树种的筛选提供理论依据。By pot experiments in greenhouse, phytoremediation and its impact factor were investigated for one-year-old Cinnamomum camphora treated with unplanted soils （control） and at three diesel concentrations （2 g·kg^-1 （L1） , 10 g·kg^-1 （L2） and 50g·kg^-1 （L3） ） to stimulate different levels of PAHs pollution. The results show that the removal rates of PAlls by C. camphora were 85.86%, 91.95% and 80.80% at L1, L2 and L3 levels, and all were higher than unplanted treatment; PAHs accumulations in the organs of C. camphora were different, at L2 and L1 levels, the accumulation of PAHs by C. camphora was in an order： leaf〉root〉stem, while it was root〉leaf〉stem at L3 levels; and the contribution rates for PAils phytoremediation by absorption and accumulation of C. camphora were 64.26%, 22.81% and 11.39% at L1, L2 and L3 levels; the bio-concentration factors became smaller with the increase of PAHs pollution, and leaf and root were the important organs for C camphora to enrich PAHs; the temperature at 5 cm soil and polyphenol oxidase were the key factors for PAHs phytoremediation by C. camphora; the fungi, actinomycetes population and phosphatase activity were suggested as the biological indicators of soil PAHs pollution. The findings could provide a theoretic evidence for screening good trees which have great effect on remediation of PAHs pollution.

关 键 词：樟树 植物修复 多环芳烃 柴油 微生物数量 酶活性 土壤温度 

分 类 号：S792.23[农业科学—林木遗传育种]