Cloning,Expression and Bioinformatic Analysis of Dorsal Gene in Delia antique  

作　　者：Huimin WEI Yujuan ZHANG Bin CHEN Shuang REN 

机构地区：[1]Institute of Entomology and Molecular Biology, Chongqing Engineering Research Center of Bioactive Substances, Chongqing Key Laboratory of Animal Biology, Chongqing Normal University

出　　处：《Plant Diseases and Pests》2012年第2期28-32,共5页植物病虫害研究（英文版）

基　　金：Supported by National Natural Science Foundation of China (30870340,31071968);Science and Technology Research Projects of Chongqing Education Commission (KJ100620 );Key Project of School Fund of Chongqing Normal University (2011XLZ12)

摘　　要：[ Objective ] The aim was to explore the relationship between the Dorsal and diapause development of Delia araiqua. [ Method ] The full-length cDNA of Dorsal in D. antiqua was cloned through RACE. The similarity among deduced amino acid sequence of Dorsal cDNA and the Dorsals of other 14 insect species were compared, and the phylogenetic analysis of these Dorsals was conducted. The expression level of Dorsal gene in winter-, summer- and non-diapausing pupae was analyzed. [ Result] The full-length of Dorsal cDNA sequence, 2 412 bp, was obtained with ORF 1974 bp, which coded 657 amino acids with predicted Mw 72.9 kDa and PI 8.5. The result of similarity comparison indicated that the DaDorsal was most related to those of Drosophlia melanogaster and Drosophlia pseudoobscura. The result of semi-quantitative RT-PCR analysis showed the expression level of Dorsal gene increased in characteristic duration of winter-, summer- and non- diapansing pupae, especially at the late diapanse, which might imply its relationship to D. antiqua diapause and development. [ Conclusion] The study lays the foundation for further study on gene function of Dorsal in insect diapause and development.[ Objective ] The aim was to explore the relationship between the Dorsal and diapause development of Delia araiqua. [ Method ] The full-length cDNA of Dorsal in D. antiqua was cloned through RACE. The similarity among deduced amino acid sequence of Dorsal cDNA and the Dorsals of other 14 insect species were compared, and the phylogenetic analysis of these Dorsals was conducted. The expression level of Dorsal gene in winter-, summer- and non-diapausing pupae was analyzed. [ Result] The full-length of Dorsal cDNA sequence, 2 412 bp, was obtained with ORF 1974 bp, which coded 657 amino acids with predicted Mw 72.9 kDa and PI 8.5. The result of similarity comparison indicated that the DaDorsal was most related to those of Drosophlia melanogaster and Drosophlia pseudoobscura. The result of semi-quantitative RT-PCR analysis showed the expression level of Dorsal gene increased in characteristic duration of winter-, summer- and non- diapansing pupae, especially at the late diapanse, which might imply its relationship to D. antiqua diapause and development. [ Conclusion] The study lays the foundation for further study on gene function of Dorsal in insect diapause and development.

关 键 词：DORSAL Delia ant/qua RACE Sequence analysis Phylogenetic analysis Semi-quantitative RT-PCR 

分 类 号：Q78[生物学—分子生物学]