东北地区11种蒲公英咖啡酸和绿原酸的含量测定  

Determination of Chlorogenic Acid and Caffeic Acid in 11 Species of Taraxacum distributed in northeast China

作　　者：宁伟[1] 贾庆飞[1] 朱丹[1] 李天来[1] 

机构地区：[1]沈阳农业大学园艺学院/东北野菜种质异位保存圃与鉴定中心/设施园艺省部共建教育部重点实验室,沈阳110161

出　　处：《沈阳农业大学学报》2012年第5期595-598,共4页Journal of Shenyang Agricultural University

基　　金：农业部项目(农计发[2010]29号)

摘　　要：比较东北地区不同种蒲公英中咖啡酸和绿原酸的含量.采用Diamonsil ODS-C18色谱柱(250mm×4.6mm,5m),以甲醇-水(含0.5%冰醋酸)系统梯度洗脱,检测波长为323nm,流速为1.0mL.min-1,柱温35℃。绿原酸Y=11892X+0.0291,r=0.9991,平均回收率(n=6)为98.9%,RSD为1.53%。回归方程:咖啡酸Y=17993X-4.6433,r=0.9996,平均回收率(n=6)为99.5%,RSD为2.58%;11种蒲公英咖啡酸和绿原酸差异较大,丹东蒲公英中咖啡酸和绿原酸含量最高,戟片蒲公英咖啡酸含量低于药典标准,为蒲公英药材的质量控制和种质资源评价提供基础性理论依据。To determine the content of caffeic acid and chlorogenic acid in Taraxacum of different species,the fingerprints were obtained from Agilent 1100 series instrument.Chromatographic experiments were performed on a Diamonsil ODS-C18（250mm×4.6 mm,5μm） analytical column,eluted with methand and water,containing 0.5% acetic acid as the mobile phases in gradient elution at the flow rate of 1.0 mL·min-1.The detection wavelength was 323nm.The temperature of column was 35℃.Results showed that chlorogenicacid Y=11892X＋0.0291,r=0.9991,the average recovery was（n=6） 98.9%,RSD为1.53%.caffeic acid Y=17993X-4.6433,r=0.9996,the average recovery was（n=6）为99.5%,RSD为2.58%.There was grate variance in the content of Chlorogenic Acid and Caffeic Acid among different species of Taraxacum.The content of T.antungense Kitag.is the highest,while T.asiaticum Dahlst.is the lower than the standard of pharmacopeia which is not suitable for herb.To some extent this study can be used to control the quality of the different kinds of Taraxacum.MoreBack AbstractFilter（＇EnChDivSummary＇,＇ChDivSummaryMore2＇,＇ChDivSummaryReset2＇）; 【Keywords in Chinese】 不同种; 蒲公英; 绿原酸; 咖啡酸; 高效液相色谱; 【Key words】 different species; Taraxacum; caffeic acid; chlorogenic acid; HPLC;

关 键 词：不同种 蒲公英 绿原酸 咖啡酸 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]