清燥救肺汤抗肺炎支原体感染大鼠作用的实验研究  

Experimental research into the effect of Qingzao Jiufei decoction on rats infected with mycoplasma pneumoniae

作　　者：吴振起[1] 刘光华[2] 岳志军[1] 南春红[1] 王子[1] 王巍[3] 朱万青[3] 

机构地区：[1]辽宁中医药大学附属医院儿科,沈阳110032 [2]辽宁中医药大学基础医学院温病教研室 [3]辽宁中医药大学研究生院

出　　处：《中国中西医结合儿科学》2013年第1期1-4,F0003,共5页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：辽宁省科技厅博士启动基金(20101067);沈阳市科技基金(F11-264-1-62);辽宁省教育厅优秀人才基金(LJQ2011102)

摘　　要：目的观察清燥救肺汤对肺炎支原体(MP)感染大鼠肺组织病理炎症及肺泡灌洗液(BALF)MP含量的影响,探讨清燥救肺汤的作用机制。方法 90只大鼠随机分正常组、模型组、清燥救肺汤高、中、低剂量组及罗红霉素组,每组各15只。后5组大鼠接种MP,制成大鼠MP感染模型;各组分别给予同容积药物或生理盐水灌胃。感染后第7、10、14天取材,对肺组织炎症程度进行评分,同时检测大鼠BALF中MP阳性率和MP-DNA的含量。结果造模第7天后,模型组肺组织病理改变、BALF中MP的阳性率和MP-DNA含量测定明显高于正常组,说明造模成功;在改善肺部炎症方面,清燥救肺汤高剂量组与罗红霉素组疗效显著,清燥救肺汤中剂量组在造模第10、14天表现较好的疗效,清燥救肺汤低剂量组则作用不明显;在降低BALF中MP-DNA方面,清燥救肺汤高剂量组与罗红霉素组均疗效显著,清燥救肺汤中剂量组在MP感染后第7天作用不明显,而在第10、14天也具有一定作用,且第7天与第14天比较有一定差异;清燥救肺汤低剂量组作用不明显。结论清燥救肺汤具有抗MP作用,可能存在一定的量效关系和时效关系。Objective To observe the effect and the mechanism of Qingzao Jiufei decoction on pathological inflammation of lung tissue and bronchoalveolar lavage fluid （BALF） in rats infected with myeoplasma pneumoniae （MP）. Methods Totally 90 rats were randomized into 6 groups, namely, normal group （group A）, model group （group B）, Qingzao Jiufei high-dose group （group C）, Qingzao Jiufei medium-close group （group D）, Qingzao Jiufei low-dose group （group E） and roxithromycin group （group F） , with 15 rats in each. The rats of the last five groups were made into MP infection models. The same volume of drugs or normal saline was given to each group by introgastric administration respectively. The pathological inflammation score of lung tissue, MP and MP- DNA of BALF was detected after 7, 10 and 14 clays of MP infection. MP of BALF was tested by polymerase chain reaction （PCR） test, and MP - DNA content was tested by fluorescent quantitative PCR （Real-time FQ PCR）. Results Seven days after MP infection models were made, the pathological changes, the positive rate and the content of MP- DNA in B group were obviously higher than those in group A, which showed that MP infection models were made successfully. In group C and group F pulmonary inflammation had obvious improvement, group D achieved effective results after 10 and 14 days of infection, group E did not achieve obvious effect. Group C, D and F had significantly more effect than other groups in reducing MP - DNA in BALF; the effect of group E was not obvious at 7^th and 10^th day, but had an advantage at 14th day. Conclusions It shows that Qingzao Jiufei decoction is effective in anti-MP, and the efficacy might be correlated with dose and time of administration and infection.

关 键 词：肺炎 支原体 清燥救肺汤 治疗应用 大鼠 动物 实验 

分 类 号：R33[医药卫生—人体生理学]