甘蔗丝氨酸/苏氨酸激酶基因的克隆及序列分析  

Molecular cloning and sequence analysis of STK from Saccharum officinarum

作　　者：陈玲[1,2,3] 叶冰莹[1,2,3] 黄贞杰[1,2,3] 张积森[1,2,3] 陈由强[1,2,3] 陈如凯 

机构地区：[1]福建师范大学生命科学学院,福建福州350108 [2]农业部福建甘蔗生物学与遗传育种重点实验室,福建福州350108 [3]发育与神经生物学福建省高等学校重点实验室,福建福州350108

出　　处：《亚热带农业研究》2012年第3期194-198,共5页Subtropical Agriculture Research

基　　金：农业部现代农业产业技术体系建设专项资金(CARS-20-4-4);福建省自然科学基金(2009J05058)资助项目

摘　　要：以福农28甘蔗品种为实验材料,电子克隆设计引物,克隆得到了甘蔗丝氨酸/苏氨酸激酶(serine/threonine kinase,STK)基因的cDNA片段。采用RACE技术克隆获得了甘蔗叶STK基因cDNA的5'及3'端序列,长度分别为750和1000bp。5'、3'端序列与中间片段重叠延伸后总片段长为1133 bp,其中5'UTR为54 bp,3'UTR为254 bp,包含完整的ORF为825 bp,共编码274个氨基酸。开放阅读框架查询器(Open Reading Frame Finder)确定其为丝氨酸/苏氨酸激酶,通过预测(//cn.expasy.org/tools)该蛋白质分子质量为29.9052 ku,理论等电点为6.36。将其基因序列与NCBI中其他植物的STK进行比对,与高粱、玉米、水稻、短柄草和大麦的相似性分别为97%、94%、89%、89%和88%。A cDNA encoding serine/threonine kinase was isolated from the leaf of Sace/mrum officinamart FN 28 with silicon cloning designing primers, 5＇ and 3＇ untranslated regions got by using RACE was 750 bp and 1000 bp respectively. The total sequence con- sists of 1133 bp after overlapping and extending the sequences of 5＇, 3＇ and middle segment, have 5＇ UTR of 54 bp, 3＇ UTR of 254 bp and an open reading frame of 825 bp that encodes the protein of 274 amino acids. Open Reading Frame Finder determine it for serine/threonine kinase, the molecular weight is 29.9052 ku and theoretical pI is 6.36 by forecasting（//cn, expasy, org/tools）. Comparing the STK gene sequence with other plants of NCBI, the similarity of Sorghum bicolor, Zea maya, Ory＇za saliva, Brachypo- dium d/stachyon, ttordetum vulgare is 97%, 94%, 89%, 89%, 88% respectively.

关 键 词：甘蔗 丝氨酸 苏氨酸激酶 基因克隆 电子克隆 序列分析 

分 类 号：Q946[生物学—植物学]