猪繁殖与呼吸综合征病毒山西分离株ORF5基因的序列分析  

Sequence Analysis of ORF5Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolated from Shanxi Area

作　　者：刘文俊[1] 姚敬明[1] 吴忻[1] 王娟萍[1] 孟帆[1] 韩一超[1] 樊振华[1] 米瑞娟[1] 罗甜甜[2] 温永亮[1] 

机构地区：[1]山西省农科院畜牧兽医研究所,山西太原030032 [2]山西省动物疫病预防控制中心,山西太原030024

出　　处：《中国畜牧兽医》2013年第2期10-13,共4页China Animal Husbandry & Veterinary Medicine

基　　金：山西省科技攻关项目(20080311036-1);山西省基础研究项目(青年科技研究基金)(2012021028-1)

摘　　要：采用RT-PCR方法对2009—2011年山西省疑似猪繁殖与呼吸综合征(PRRS)阳性病料进行克隆和测序,获得5个ORF5基因片段,并对其基因序列和推导的氨基酸序列与国内外毒株进行了同源性分析。序列分析结果表明,5个ORF5基因序列之间核苷酸同源性为97.7%～98.5%,与欧洲型代表株LV、美洲型代表株VR-2332、2006—2007年国内分离的PRRSV变异株(JXA1、HuN、HUN4、HUB1)、2006年以前中国分离毒株(CH-1a、HB-1(sh)、HB-2(sh))和2个疫苗株(RespPRRS MLV、MLV RespPRRS/Repro)核苷酸同源性分别为63.5%～64.0%、88.7%～89.2%、97.7%～99.3%、88.1%～96.7%和88.6%～89.1%;氨基酸同源性分别为56.1%～56.6%、87.8%～88.8%、96.6%～98.5%、85.9%～93.2%、86.8%～87.9%。结果表明山西地区的PRRSV流行毒株均属于美洲型,且毒株同源性很高,亲缘关系紧密。Through RT-PCR amplification of tissue or blood sample from Shanxi province, the 50RF5 gene nucleotide sequences were obtained. The obtained sequences and the deduced amino acid were analyzed and compared with the other pub-lished PRRSV strains. The nucleotide homology were 97.7 % to 98.5 % among 50RF5,comparing with LV, the strains isolated from China in 2006 to 2007, the strains isolated before 2006, Resp PRRS MLV and MLV RespPRRS/Repro, the nucleotide homology were 63.5% to 64.00%0, 88.7% to 89.2%, 97.7% to 99.3%, 88.1% to 96.7%, 88.6% to 89.1%. The aniniacid homology were 56.1% to 56.6%,87.8% to 88.8%,96.6% to 98.5%,85.9% to 93.2%,86.8% to 87.9%, respectively. The results showed that the prevalent PRRSV strains in Shanxi belong to North American genotype which had high homology and correlative genetic relationship.

关 键 词：猪繁殖与呼吸综合征病毒 ORF5基因 变异分析 

分 类 号：Q78[生物学—分子生物学]