叉头框转录因子O亚族1基因对猪肾周脂肪沉积代谢的影响  

Effect of Forkhead Box Transcription Factor O1 Gene on the Perirenal Fat Metabolism of the Pigs

作　　者：张野[1] 崔世泉[1] 狄生伟[1] 黄宣凯[1] 王希彪[1] 

机构地区：[1]东北农业大学动物科学技术学院,黑龙江哈尔滨150030

出　　处：《中国畜牧兽医》2013年第2期117-120,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划项目(2008BADB2B02-5)

摘　　要：叉头框转录因子O亚族1(forkhead box transcription factor O1,FoxO1)在细胞增殖、分化过程中发挥着重要的作用。采用实时荧光定量PCR技术,对不同月龄民猪和长白猪肾周脂肪中FoxO1、过氧化物酶体增殖物激活受体(peroxisomeproliferator-activated receptorγ,PPARγ)和CAAT区/增强子结合蛋白α(CAAT/enhancer binding proteinα,C/EBPα)基因mRNA表达量进行检测,探讨FoxO1在猪肾周脂肪代谢过程中的作用。结果显示,2品种猪肾周脂肪2.5月龄FoxO1基因mRNA表达量极显著高于1、6、8月龄(P<0.01);分析FoxO1与PPARγ、C/EBPα基因的表达关系,结果发现在2.5月龄后,FoxO1mRNA的表达与PPARγ、C/EBPα相反,说明FoxO1可能通过抑制这2个基因的表达来减少脂肪沉积。Forkhead box transcription factor O1 （FoxO1） played a critical role in cell proliferation and differentiation of mammals. An experiment was conducted to determine the mRNA expression of FoxO1 ,PPAR 7 and C/EBPa from perirenal fat of pigs by using Real-time PCR to study the role of FoxO1 in fat metabolism. The results indicated that in perirenal adi- pose,the expression of FoxO1 mRNA in Min pigs and Landrace of 2.5 months of age was significant higher than that in pigs of 1,6 and 8 months of age （P〈0.01）. According to the analysis of the relationship between mRNA expression of FoxO1 and PPAR7, C/EBPa gene, the expression trend of FoxO1 was contrary to PPARγ,C/EBPa after 2 months, which manifested that FoxO1 inhibited fat deposition through inhibiting PPARγ, C/EBPa mRNA expression. The results suggested that FoxO1 played an inhibitting role in fat deposition of pigs.

关 键 词：FOXO1 PPARΓ C EBPα 实时荧光定量PCR 肾周脂肪 

分 类 号：Q786[生物学—分子生物学]