肉鸭中黄病毒的分离与鉴定  

Isolation and Identification of Duck Flavivirus

作　　者：赖芳芳[1] 陈孝明 张健騑[3] 罗均 陈善真 王贵平 李中圣 

机构地区：[1]华南农业大学,广东广州510642 [2]广东现代农业集团研究院,广东广州510630 [3]广东省农业科学院兽医研究所,广东广州510640

出　　处：《中国畜牧兽医》2013年第2期179-182,共4页China Animal Husbandry & Veterinary Medicine

基　　金：广东省科技厅项目(2011A090400011)

摘　　要：为探索2011年年底在广东阳江地区某肉鸭场出现的病鸭和死鸭的发病原因,对其中2个发病鸭场的病死鸭进行了解剖,对病变组织的样品应用RT-PCR的方法进行了病原鉴定。经剖检,发现鸭心脏表面形成小结节,伴有少量心包积液;肝脏和胆囊肿大;脾脏有花斑;胰腺和直肠有出血点。利用病料研磨上清和病料接种后的鸡胚尿囊液进行RT-PCR检测,结果显示鸭黄病毒阳性。序列分析显示,该病毒的核苷酸序列与坦布苏病毒Fengxian株的相似性为99%。试验结果表明,该病毒为黄病毒,是引起此次鸭场发病的病原。At the end of 2011, a new duck infectious disease emerged in Guangdong province. In order to identify the etio-logical agent, the pathological examination and RT-PCR were carried out. By autopsy, we found that cardiac sarcoidosis, accompanied with a few pericardial effusion; liver and gallbladder swelled; spleen was slightly mottled; pancreas and rectum with hemorrhagic spots. The samples from affected ducks and the allantoie fluid of affected chick embryos, were detected positive by duck flavivirus RT-PCR method. Sequence analysis showed that the isolated virus shared 99% nucleotide identity with duck flavivirus Fengxian strain. These results suggested that the isolated virus belonged to duck flavivirus and was the reason for the infectious disease of duck.

关 键 词：鸭黄病毒 RT-PCR 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]