5种瓜类植物多聚半乳糖醛酸酶抑制蛋白的诱导表达及活性测定  

Induced expression and activity detection of Polygalacturonaseinhibiting protein in 5 kinds of cucurbits crops

作　　者：李建湘[1] 王振中[1] 

机构地区：[1]华南农业大学资源环境学院,广东广州510642

出　　处：《广东农业科学》2013年第1期143-146,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(30971888);国家公益性行业(农业)科研专项(200903049)

摘　　要：多聚半乳糖醛酸酶抑制蛋白(PGIP)是一种富含亮氨酸重复单位的糖蛋白,能非竞争性地抑制真菌多聚半乳糖醛酸酶(PG)的活性,从而提高植物的抗病性。以黄瓜、苦瓜、冬瓜、节瓜和甜瓜幼苗为材料,研究PGIP的诱导表达特性及在不同组织中的含量差异。通过比较经孢子诱导、SA诱导和黑暗诱导的黄瓜幼苗中的PGIP的相对含量,发现经病原菌孢子悬浮液处理48 h时PGIP的表达量最高。对经孢子诱导48 h的黄瓜幼苗的根、茎、叶中的PGIP含量进行比较,发现茎中PGIP的相对含量最高。测定这5种瓜类的PGIP对6种尖孢镰刀菌粗PG的抑制作用,发现苦瓜PGIP的相对活性较高,并证实了不同的PGIP能够特异性地识别不同的PG,PGIP对不同PG存在着选择性抑制。Polygalacturonase-inhibiting protein （PGIP） is a leucine-fich repeat cell wall-binding protein that can inhibit the activity of fungal PG specifically and non-competitively, thus improve the disease resistance of plants. Cucurbitaceae cucumis, Momordica charantia, Benincasa hispida, Benincasa hispida var. chieh-qua and Cucumis melo seedlings were selected in this research. Biotic stressed plants of C. cucumis showed high induced expression activity, rose up to maximum at 48 h and stems showed maximum expression than other organs. Biotic stressed plants of 5 kinds of cucurbits crops are used to prepare crude inhibitor and their activity was checked by inhibition assay to 6 kinds of PG respectively, PGIP of M. charantia showed high activity than others PGIP. The results showed a wide spectrum of activity of PGIP against different PG and there might be selective inhibition.

关 键 词：瓜类枯萎病 尖孢镰刀菌 多聚半乳糖醛酸酶 多聚半乳糖醛酸酶抑制蛋白 

分 类 号：S432.1[农业科学—植物病理学]