棉花水通道蛋白序列生物信息学初步分析  

Preliminary bioinformatics analysis of aquaporin sequences available in cotton

作　　者：王为[1,2] 王长彪[3] 陈浩东[1,4] 刘方[1] 王春英[1] 张香娣[1] 王玉红[1] 王坤波[1] 

机构地区：[1]中国农业科学院棉花研究所,棉花生物学国家重点实验室,河南安阳455000 [2]江苏沿海地区农业科学研究所,农业部沿海盐碱地科学观测实验站,江苏盐城223802 [3]山西省农业科学院棉花研究所,山西运城043800 [4]湖南省棉花科学研究所国家杂交棉研究推广中心,湖南常德415101

出　　处：《浙江农业学报》2013年第1期14-20,共7页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(30900911,31000729);中央级公益性科研院所基本科研业务专项(SJB1105);江苏省科技支撑计划(BE2011304);江苏沿海所所长基金(YHS201103);江苏省农业科技自主创新资金[CX(12)5073]

摘　　要：水通道蛋白(aquaporin,AQP)是高效转运水分子的膜内在蛋白,在调控植物的水分关系中有重要作用,和抗旱性能紧密相关。为初步评价分析棉花AQP序列资源,对网上最新且仅有的52条棉花AQP序列进行分子聚类,同时将其进行多序列比对及亚细胞定位,结果表明:棉花AQP大致分为7类,具有丰富的多样性,AQP基因在跨膜转运以及光合中起重要作用。另外,来自水稻、大豆、玉米、拟南芥的328条AQP序列和52条棉花AQP序进行分子聚类,结果表明:有48条棉花AQP和大豆聚为一类,遗传距离较接近。Aquaporin （AQP） is a kind of inner epicyte protein which efficiently transports water molecule relevant to drought resistance and plays an important role in water regulation. To evaluate and analyze cotton AQP sequence re- sources preliminarily, the latest 52 AQP sequences from cotton public database were selected and analyzed by molec- ular clustering, multi-sequence alignment and distribution and location of AQP genes in subtype cell. The results in- dicated that AQPs were roughly divided into seven categories with a rich diversity, and AQP genes played an impor- tant role in the transportation through membrane and photosynthesis. Besides, 328 AQP sequences from rice, soy- bean, maize, Arabidopsis and the latest online 52 AQP sequences available in cotton were carried out with molecular clustering. The results showed that 48 out of the 52 AQP sequences in cotton and soybean gathered for a clustering with a closer genetic distance.

关 键 词：棉花 水通道蛋白 耐盐 抗旱 分子聚类 

分 类 号：S562[农业科学—作物学]