分子修饰后枸杞多糖对睾丸生精功能保护作用  

Protective Effects of Molecularly Modified Lycium Barbarum Polysaccharides on Testicular Spermatogenic Function of Rats

作　　者：李环[1] 张晶[1] 赵宏伟 于敬红[1] 

机构地区：[1]北华大学公共卫生学院,吉林吉林132013 [2]金华市浦江县疾病控制中心,浙江金华322204

出　　处：《北华大学学报（自然科学版）》2013年第1期35-38,共4页Journal of Beihua University（Natural Science）

基　　金：吉林省科技发展计划项目(201205038);北华大学大学生国家级创新项目(101020135)

摘　　要：目的观察硒化枸杞多糖对邻苯二甲酸二丁酯(DBP)所致的雄性大鼠睾丸组织氧化损伤的保护作用,并确定枸杞硒多糖的最佳反应条件.方法在醋酸催化作用下,利用亚硒酸钠对枸杞多糖进行分子修饰.将48只健康雄性SD大鼠随机分成对照组、DBP+硒化枸杞多糖组、DBP+硒组、DBP+枸杞多糖组、DBP染毒组,连续灌胃30 d,检测睾丸组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)和丙二醛(MDA)的含量.结果硒化枸杞多糖的最佳条件是室温(20℃),亚硒酸钠与枸杞多糖用量为1 1,反应时间为48 h.硒化枸杞多糖组与除对照组的其他组比较脂质过氧化指标均有统计学意义(P<0.05);同时硒化枸杞多糖可显著提高体内SOD,T-AOC以及GSH-Px含量,降低MDA水平.结论硒化修饰枸杞多糖后的抗氧化作用要强于普通枸杞多糖和无机硒.Objective To observe the protective effect of selenium lyceum barbarum polysaccharides （LBP）on dibutyl phthalate （DBP）-induced male rat testicular oxidative tissue damage and determine the best reaction condition for obtaining selenium LBP. Method On the basis of the catalytic effect of acetic acid, sodium selenite was used for the molecular modification of LBP. 48 Healthy male SD rats were divided into control group, DBP ＋ selenium group, LBP group, DBP ＋ selenium group, DBP ＋ LBP group and DBP-infected group at random. They were given the corresponding agents in intragastric administration for 30 days. Contents of SOD, glutathione peroxidase （ GSH-Px）, total antioxidant capacity （ T-AOC ） and malondialdehyde （MDA） in testicular tissues of the rats were measured. Results The best conditions for making the selenium LBP included that the best temperature was room temperature （20 ~C ）, the proportion of sodium selenite and LBP was 1 ： 1, and the reaction time was 48 h. Compared with those in the other groups except the control group, the lipid peroxidation indexes of rats in selenium LBP improve the contents group were different significantly （ P 〈 0.05）. Moreover, selenium LBP could significantly of SOD, T-AOC and GSH-Px, and lower the MDA level in the body. Conclusion The antioxidant effect of the selenium LBP is superior to that of normal LBP or selenium.

关 键 词：硒化枸杞多糖 邻苯二甲酸二乙酯 抗氧化 

分 类 号：R285[医药卫生—中药学]