急性肾衰竭动物模型的复制及实验性治疗  

Copy of Acute Renal Failure in Rabbits and Experimental Treatment

作　　者：葛贺[1] 李冰[1] 黄晓莉[1] 杨丽娟[1] 陈立松[1] 芦丽莉[1] 刘艳波[1] 

机构地区：[1]北华大学基础医学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2013年第1期39-42,共4页Journal of Beihua University（Natural Science）

基　　金：吉林省科技发展计划项目(2009011;2012009)

摘　　要：目的复制家免急性肾衰竭动物模型并进行实验性治疗,为本科学生综合性实验的实施奠定基础.方法通过肌肉注射50%甘油(10 mL/kg)复制家兔ARF模型,各组动物进行心电图监测,治疗组通过颈外静脉滴注碳酸氢钠溶液、呋塞米溶液及CaCl2溶液进行治疗,对比观察24 h后血清尿素氮(BUN)、血肌酐(Cre)水平、pH变化、尿量及尿肌酐变化.结果 50%甘油肌肉注射可复制出明显的急性肾衰的动物模型,模型组动物血清中BUN,Cre水平明显高于阴性对照组,其尿肌酐含量较高,每分钟尿量明显减少,并出现明显的代谢性酸中毒;而治疗组动物血清BUN,Cre水平明显低于模型组,其尿肌酐含量减少,每分钟尿量明显多于模型组,酸中毒缓解.模型组动物血压偏高,但与阴性对照组无明显差别,而心电图发现T波高耸,QRS波增宽.治疗后其心电图逐渐恢复正常.结论肌肉注射50%甘油复制急性肾衰模型的方法可靠,可作为综合性实验教学方法在基础实验教学中广泛应用.Objective To copy the acute renal failure （ARF）animal model and execute an experimental treatment of it in rabbits, to lay a foundation for the implementation of comprehensive experiment for undergraduate students. Method ARF model was set up through the injection of 50% glycerol （ 10 mL/kg）. All the rabbits were monitored with ECG, Sodium bicarbonate solution, furosemide solution and CaC1z solution were infused through the external jugular vein to the rabbits in the treatment group. Serum urea nitrogen （ BUN ）, serum creatinine （Cre） level, plasma pH, urine volume and urine creatinine changes of the rabbits were detected after 24 hours. Results The application of 50% glycerol intramuscular injection could replicate typical animal models with acute renal failure. Serum BUN and Cre levels concentration in the model group were obviously higher than those in the negative control group, the urine Cre concentration was also higher, while the urine volume per minute was less and a metabolic acidosis happened at the same time. In the treatment group, serum BUN and Cre levels were significantly lower than those in the model group, while urine volume per minute was more than that in the control group, and the metabolic acidosis was improved. The blood pressure of animals in the treatment group was a litter higher, but there was no difference compared with that in the negative control group, T wave was high and giant, and the QRS wave was widened in the model group compared with those in the negative control group. After the treatment, ECG of the animals in the model group gradually returned to normal state. Conclusion The method of intramuscular injection of 50% glycerol could be reliable to replicate the animal model with acute renal failure, and can be taken as a comprehensive experimental teaching method and widely used in the teaching of medical functional experiment.

关 键 词：急性肾衰竭 甘油 综合性实验 

分 类 号：R586[医药卫生—内分泌]