生血灵对激素抵抗型免疫性血小板减少症蛋白质表达的影响  

The Effect of Sheng-xue-ling on the Protein Expression of Steroid-resistant ITP Patients

作　　者：郭锦荣[1] 鲍计章[1] 周永明[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院

出　　处：《山西中医》2013年第3期40-42,共3页Shanxi Journal of Traditional Chinese Medicine

基　　金：上海市科学技术委员会科研项目(编号:10DZ1974100);上海市科学技术委员会科研项目(编号:11DZ1971600);上海市卫生局科研项目(编号:2011ZJ018);上海市卫生局青年基金项目(编号:2008Q002L);上海市中医药事业三年行动计划首批重大研究项目(编号:ZYNXD-CC-ZDYJ020)

摘　　要：目的:观察生血灵对激素抵抗型免疫性血小板减少症(ITP)患者蛋白质表达的影响及其作用机制。方法:采用双向电泳、质谱分析等蛋白质组学技术建立ITP患者、激素敏感型ITP患者血清全蛋白双向凝胶电泳(2-DE)图谱,分析各组之间差异的蛋白质位点,并观察生血灵对激素抵抗型ITP患者蛋白质表达的调节作用。结果:ITP激素抵抗型患者蛋白质纤维二糖水解酶的高表达、外切纤维二糖水解酶的低表达,与激素敏感型患者存在显著差异(P<0.05)。经生血灵治疗后,蛋白质表达水平有所改善,与治疗前相比具有显著性差异(P<0.01)。结论:ITP激素抵抗型患者纤维二糖水解酶相对高表达、外切纤维二糖水解酶相对低表达,它可能是ITP患者产生激素抵抗的重要机制,也是激素治疗无效的重要原因;中药生血灵通过调节蛋白质表达水平而逆转激素抵抗,提高临床疗效。Objective ： To observe the effect of Sheng-xue-ling on protein expression in steroid-resistant immune thrombocytopenia （ITP） as well as its mechanism of action. Methods： With such proteomics technology as two-di- mensional electrophoresis and mass spectrometry it was established who were＇the profiles of serum total protein two- dimensional gel electrophoresis （2-DE） of the ITP patients and of the steroid-resistant ITP patients. Then the difference of protein loci was analyzed between each group. And the effect of Sheng-xue-ling was observed on pro- tein expression in steroid-resistant immune thrombocytopenia （ITP） patients. Results： Among steroid-resistant ITP patients the protein expression of cellobiose hydrolytic enzymes was high, and that of exocellobiohydrolase was low. There was significant difference between the ITP patients and the steroid-resistant on them （P 〈 0. 05 ）. After treatment with sheng-xue-ling, the protein expressions were improved, and a significant difference showed up com- pared with before treatment （P 〈 0. 01 ）. Conclusion ： The high protein expression of cellobiose hydrolytic enzymes and the low protein expression of exocellobiohydrolase could be the important action mechanism of hormonal resist- ance in ITP patients, which could be also the important cause of ineffective hormone therapy. Sheng-xue-ling can improve the clinical efficacy through regulating the protein expression and the reversal of hormone resistance. Shengxue Ling by regulating the protein expression level and, improve the clinical efficacy.

关 键 词：免疫性血小板减少症 生血灵 蛋白质 临床研究 

分 类 号：R558.2[医药卫生—血液循环系统疾病]